N-Glycosylation Modification of Butyrylcholinesterase in a Transgenic Rice CellCulture

N-Glycosylation Modification of Butyrylcholinesterase in a Transgenic Rice CellCulture

Propofol Impacts Non-Small-Cell Lung Most cancers Cell Biology By Regulating the miR-21/PTEN/AKT Pathway In Vitro and In Vivo 

Background: Propofol is a typical sedative-hypnotic drug historically used for inducing and sustaining basic anesthesia. Current research have drawn consideration to the nonanesthetic results of propofol, however the potential mechanism by which propofol suppresses non-small-cell lung most cancers (NSCLC) development has not been absolutely elucidated.

Strategies: For the in vitro experiments, we used propofol (0, 2, 5, and 10 µg/mL) to deal with A549 cells for 1, 4, and 12 hours and Cell Counting Package-8 (CCK-8) to detect proliferation. Apoptosis was measured with circulation cytometry. We additionally transfected A549 cells with an microribonucleic acid-21 (miR-21) mimic or damaging management ribonucleic acid (RNA) duplex and phosphatase and tensin homolog, deleted on chromosome 10 (PTEN)

small interfering ribonucleic acid (siRNA) or damaging management. PTEN, phosphorylated protein kinase B (pAKT), and protein kinase B (AKT) expression have been detected utilizing Western blotting, whereas miR-21 expression was examined by real-time polymerase chain response (RT-PCR). In vivo, nude mice got injections of A549 cells to develop xenograft tumors; Eight days later, the mice have been intraperitoneally injected with propofol (35 mg/kg) or soybean oil. Tumors have been then collected from mice and analyzed by immunohistochemistry and Western blotting.

Outcomes: Propofol inhibited development (1 hour, P = .001; Four hours, P ≤ .0001; 12 hours, P = .0004) and miR-21 expression (P ≤ .0001) and induced apoptosis (1 hour, P = .0022; Four hours, P = .0005; 12 hours, P ≤ .0001) in A549 cells in a time and concentration-dependent method. MiR-21 mimic and PTEN siRNA transfection antagonized the suppressive results of propofol on A549 cells by lowering PTEN protein

expression (imply variations [MD] [95% confidence interval {CI}], -0.51 [-0.86 to 0.16], P = .0058; MD [95% CI], 0.81 [0.07-1.55], P = .0349, respectively), leading to a rise in pAKT ranges (MD [95% CI] = -0.82 [-1.46 to -0.18], P = .0133) following propofol publicity. In vivo, propofol therapy diminished NSCLC tumor development (MD [95% CI] = -109.47 [-167.03 to -51.91], P ≤ .0001) and promoted apoptosis (MD [95% CI] = 38.53 [11.69-65.36], P = .0093).

Conclusions: Our research indicated that propofol inhibited A549 cell development, accelerated apoptosis by way of the miR-21/PTEN/AKT pathway in vitro, suppressed NSCLC tumor cell development, and promoted apoptosis in vivo. Our findings present new implications for propofol in most cancers remedy and point out that propofol is extraordinarily advantageous in surgical therapy.

Conditional reprogramming: subsequent expertise cell tradition

Prolonged-term main custom of mammalian cells has been always robust attributable to unavoidable senescence. Commonplace methods for producing immortalized cell strains usually require manipulation of genome which leads to change of important natural and genetic traits. Not too way back, conditional reprogramming (CR) emerges as a novel subsequent expertise instrument for long-term custom of main epithelium cells derived from practically all origins with out alteration of genetic background of main cells.

CR co-cultures main cells with inactivated mouse 3T3-J2 fibroblasts inside the presence of RHO-related protein kinase (ROCK) inhibitor Y-27632, enabling main cells to build up stem-like traits whereas retain their potential to fully differentiate. With just some years’ development, CR displays broad prospects in functions in diversified areas along with sickness modeling, regenerative medicine, drug evaluation, drug discovery along with precision medicine. This consider is thus to comprehensively summarize and assess current progress in understanding mechanism of CR and its broad functions, highlighting the value of CR in every main and translational researches and discussing the challenges confronted with CR.

Outcomes of Kifunensine on Manufacturing and N-Glycosylation Modification of Butyrylcholinesterase in a Transgenic Rice CellCulture Bioreactor

The manufacturing and N-glycosylation of recombinant human butyrylcholinesterase (BChE), a model extraordinarily glycosylated therapeutic protein, in a transgenic rice cell suspension custom dealt with with kifunensine, a strong α-mannosidase I inhibitor, was studied in a 5 L bioreactor.

A media commerce was carried out at day 7 of cultivation by eradicating spent sugar-rich medium (NB+S) and together with current sugar-free (NB-S) medium to induce the rice α-amylase 3D (RAmy3D) promoter to supply rice recombinant human BChE (rrBChE). Using a 1.25X-concentrated sugar-free medium together with an 80% lowered working amount by means of the media commerce led to an entire energetic rrBChE manufacturing diploma of 79 ± 2 µg (g FW)-1 or 7.5 ± 0.4 mg L-1 inside the presence of kifunensine, which was 1.5-times larger than our earlier bioreactor runs using common sugar-free (NB-S) media with no kifunensine treatment.

Importantly, the amount of secreted energetic rrBChE in custom medium was enhanced inside the presence of kifunensine, comprising 44% of your complete energetic rrBChE at day 5 following induction. Coomassie-stained SDS-PAGE gel and Western blot analyses revealed fully completely different electrophoretic migration of purified rrBChE bands with and with out kifunensine treatment, which was attributed to fully completely different N-glycoforms. N-Glycosylation analysis confirmed significantly elevated oligomannose glycans (Man5/6/7/8) in rrBChE dealt with with kifunensine as compared with controls. Nonetheless, the mass-transfer limitation of kifunensine was seemingly the principle function for incomplete inhibition of α-mannosidase I on this bioreactor study.

stemcelldocs
stemcelldocs
CMV control lentivirus (Hygro)
CMV-Null-Hygro 1 x107 IFU/ml x 200ul
EUR 349
Description: Negative control lentivirus contains a null spacer insert under CMV promoter, serves as the negative control of lentivurs treatment for the specificity of any target expression effects. It has the hygromycin selection under RSV promoter.
CMV control lentivirus (Zeo)
CMV-Null-Zeo 1 x107 IFU/ml x 200ul
EUR 349
Description: Negative control lentivirus contains a null spacer insert under CMV promoter, serves as the negative control of lentivurs treatment for the specificity of any target expression effects. It has the Zeocin selection under RSV promoter.
GFP Expressing Human Glioblastoma Cells
TR01-GFP 500,000 Cells
EUR 1354
GFP Expressing Human Gastric Carcinoma N87 Cells
TR02-GFP 500,000 Cells
EUR 1354
GFP Expressing Human Renal Adenocarcinoma Cells (ACHN)
TR04-GFP 500,000 Cells
EUR 1354
GFP Expressing Human Prostate Carcinoma Cells (DU 145)
TR03-GFP 500,000 Cells
EUR 1354
Lenti-HOXA10-GFP Lentivirus
LV646 10 ml
EUR 811
Lenti-HOXB8-GFP Lentivirus
LV634 10 ml
EUR 811
Lenti-HOXA9-GFP Lentivirus
LV640 10 ml
EUR 811
ISRE-Luc (GFP) Lentivirus 
LVP939-G 1x107 IFU/ml x 200ul
EUR 552
Description: Pre-made lentivirus express Firefly luciferase reporter undera minimal promoter embeded 8 tandem repeats of ISRE (Interferon Stimulated Response Element). This lentivirus also contain the GFP selection marker under the consitutiveRSV promoter, provided in DMEM medium with 10% FBS and 60ug/ml of polybrene.
ISRE-Rluc (GFP) Lentivirus 
LVP940-G 1x107 IFU/ml x 200ul
EUR 552
Description: Pre-made lentivirus express Renilla luciferase reporter undera minimal promoter embeded 8 tandem repeats of ISRE (Interferon Stimulated Response Element). This lentivirus also contain the GFP selection marker under the consitutive RSV promoter, provided in DMEM medium with 10% FBS and 60ug/ml of polybrene.
CRE_TRE-GFP (Bsd) Lentivirus 
LVP941-B 1x107 IFU/ml x 200ul
EUR 552
Description: Pre-made lentivirus express GFP reporter under a minimal promoter embeded 8 tandem repeats of cAMP response motif (CRE). This lentivirus also contain the Blasticidin selection marker under RSV promoter, provided in DMEM medium with 10% FBS and 60ug/ml of polybrene.
CRE_TRE-GFP (Neo) Lentivirus 
LVP941-N 1x107 IFU/ml x 200ul
EUR 552
Description: Pre-made lentivirus express GFP reporter under a minimal promoter embeded 8 tandem repeats of cAMP response motif (CRE). This lentivirus also contain the Neomycin selection marker under RSV promoter, provided in DMEM medium with 10% FBS and 60ug/ml of polybrene.
CRE_TRE-GFP (Puro) Lentivirus 
LVP941-P 1x107 IFU/ml x 200ul
EUR 552
Description: Pre-made lentivirus express GFP reporter under a minimal promoter embeded 8 tandem repeats of cAMP response motif (CRE). This lentivirus also contain the puromycin selection marker under RSV promoter, provided in DMEM medium with 10% FBS and 60ug/ml of polybrene.
CRE_TRE-GFP (RFP) Lentivirus 
LVP941-R 1x107 IFU/ml x 200ul
EUR 552
Description: Pre-made lentivirus express GFP reporter under a minimal promoter embeded 8 tandem repeats of cAMP response motif (CRE). This lentivirus also contain the RFP selection marker under the constitutive RSV promoter, provided in DMEM medium with 10% FBS and 60ug/ml of polybrene.
CRE_TRE-RFP (GFP) Lentivirus 
LVP942-G 1x107 IFU/ml x 200ul
EUR 552
Description: Pre-made lentivirus express RFP reporter under a minimal promoter embeded 8 tandem repeats of cAMP response motif (CRE). This lentivirus also contain the GFP selection marker under the consitutive RSV promoter, provided in DMEM medium with 10% FBS and 60ug/ml of polybrene.
CRE_TRE-Luc (GFP) Lentivirus 
LVP943-G 1x107 IFU/ml x 200ul
EUR 552
Description: Pre-made lentivirus express Firefly luciferase reporter under a minimal promoter embeded 8 tandem repeats of cAMP response motif (CRE). This lentivirus also contain the GFP selection marker under the consitutiveRSV promoter, provided in DMEM medium with 10% FBS and 60ug/ml of polybrene.
CRE_TRE-Rluc (GFP) Lentivirus 
LVP944-G 1x107 IFU/ml x 200ul
EUR 552
Description: Pre-made lentivirus express Renilla luciferase reporter under a minimal promoter embeded 8 tandem repeats of cAMP response motif (CRE). This lentivirus also contain the GFP selection marker under the consitutive RSV promoter, provided in DMEM medium with 10% FBS and 60ug/ml of polybrene.
Gli-GFP (Bsd) Lentivirus 
LVP945-B 1x107 IFU/ml x 200ul
EUR 552
Description: Pre-made lentivirus express GFP reporter under a minimal promoter embeded 8 tandem repeats of Gli responsive element (hedgehog pathway). This lentivirus also contain the Blasticidin selection marker under RSV promoter, provided in DMEM medium with 10% FBS and 60ug/ml of polybrene.
Gli-GFP (Neo) Lentivirus 
LVP945-N 1x107 IFU/ml x 200ul
EUR 552
Description: Pre-made lentivirus express GFP reporter under a minimal promoter embeded 8 tandem repeats of Gli responsive element (hedgehog pathway). This lentivirus also contain the Neomycin selection marker under RSV promoter, provided in DMEM medium with 10% FBS and 60ug/ml of polybrene.
Gli-GFP (Puro) Lentivirus 
LVP945-P 1x107 IFU/ml x 200ul
EUR 552
Description: Pre-made lentivirus express GFP reporter under a minimal promoter embeded 8 tandem repeats of Gli responsive element (hedgehog pathway). This lentivirus also contain the puromycin selection marker under RSV promoter, provided in DMEM medium with 10% FBS and 60ug/ml of polybrene.
Gli-GFP (RFP) Lentivirus 
LVP945-R 1x107 IFU/ml x 200ul
EUR 552
Description: Pre-made lentivirus express GFP reporter under a minimal promoter embeded 8 tandem repeats of Gli responsive element (hedgehog pathway). This lentivirus also contain the RFP selection marker under the constitutive RSV promoter, provided in DMEM medium with 10% FBS and 60ug/ml of polybrene.
Gli-RFP (GFP) Lentivirus 
LVP946-G 1x107 IFU/ml x 200ul
EUR 552
Description: Pre-made lentivirus express RFP reporter under a minimal promoter embeded 8 tandem repeats of Gli responsive element (hedgehog pathway). This lentivirus also contain the GFP selection marker under the consitutive RSV promoter, provided in DMEM medium with 10% FBS and 60ug/ml of polybrene.
Gli-Luc (GFP) Lentivirus 
LVP947-G 1x107 IFU/ml x 200ul
EUR 552
Description: Pre-made lentivirus express Firefly luciferase reporter under a minimal promoter embeded 8 tandem repeats of Gli responsive element (hedgehog pathway). This lentivirus also contain the GFP selection marker under the consitutiveRSV promoter, provided in DMEM medium with 10% FBS and 60ug/ml of polybrene.
Gli-Rluc (GFP) Lentivirus 
LVP948-G 1x107 IFU/ml x 200ul
EUR 552
Description: Pre-made lentivirus express Renilla luciferase reporter under a minimal promoter embeded 8 tandem repeats of Gli responsive element (hedgehog pathway). This lentivirus also contain the GFP selection marker under the consitutive RSV promoter, provided in DMEM medium with 10% FBS and 60ug/ml of polybrene.
G_RE-GFP (Bsd) Lentivirus 
LVP949-B 1x107 IFU/ml x 200ul
EUR 552
Description: Pre-made lentivirus express GFP reporter under a minimal promoter embedded 4 tandem repeats of glucocorticoid response element (G-RE). This lentivirus also contain the Blasticidin selection marker under RSV promoter, provided in DMEM medium with 10% FBS and 60ug/ml of polybrene.
G_RE-GFP (Neo) Lentivirus 
LVP949-N 1x107 IFU/ml x 200ul
EUR 552
Description: Pre-made lentivirus express GFP reporter under a minimal promoter embedded 4 tandem repeats of glucocorticoid response element (G-RE). This lentivirus also contain the Neomycin selection marker under RSV promoter, provided in DMEM medium with 10% FBS and 60ug/ml of polybrene.
G_RE-GFP (Puro) Lentivirus 
LVP949-P 1x107 IFU/ml x 200ul
EUR 552
Description: Pre-made lentivirus express GFP reporter under a minimal promoter embedded 4 tandem repeats of glucocorticoid response element (G-RE). This lentivirus also contain the puromycin selection marker under RSV promoter, provided in DMEM medium with 10% FBS and 60ug/ml of polybrene.
G_RE-GFP (RFP) Lentivirus 
LVP949-R 1x107 IFU/ml x 200ul
EUR 552
Description: Pre-made lentivirus express GFP reporter under a minimal promoter embedded 4 tandem repeats of glucocorticoid response element (G-RE). This lentivirus also contain the RFP selection marker under the constitutive RSV promoter, provided in DMEM medium with 10% FBS and 60ug/ml of polybrene.
G_RE-RFP (GFP) Lentivirus 
LVP950-G 1x107 IFU/ml x 200ul
EUR 552
Description: Pre-made lentivirus express RFP reporter under a minimal promoter embedded 4 tandem repeats of glucocorticoid response element (G-RE). This lentivirus also contain the GFP selection marker under the consitutive RSV promoter, provided in DMEM medium with 10% FBS and 60ug/ml of polybrene.
G_RE-Luc (GFP) Lentivirus 
LVP951-G 1x107 IFU/ml x 200ul
EUR 552
Description: Pre-made lentivirus express Firefly luciferase reporter under a minimal promoter embedded 4 tandem repeats of glucocorticoid response element (G-RE). This lentivirus also contain the GFP selection marker under the consitutiveRSV promoter, provided in DMEM medium with 10% FBS and 60ug/ml of polybrene.
G_RE-Rluc (GFP) Lentivirus 
LVP952-G 1x107 IFU/ml x 200ul
EUR 552
Description: Pre-made lentivirus express Renilla luciferase reporter under a minimal promoter embedded 4 tandem repeats of glucocorticoid response element (G-RE). This lentivirus also contain the GFP selection marker under the consitutive RSV promoter, provided in DMEM medium with 10% FBS and 60ug/ml of polybrene.
AP1-GFP (Bsd) Lentivirus 
LVP953-B 1x107 IFU/ml x 200ul
EUR 552
Description: Pre-made lentivirus express GFP reporter under a minimal promoter that embedded 8 tandem repeats of AP1 Responsive Element (AP1-RE) . This lentivirus also contain the Blasticidin selection marker under RSV promoter, provided in DMEM medium with 10% FBS and 60ug/ml of polybrene.
AP1-GFP (Neo) Lentivirus 
LVP953-N 1x107 IFU/ml x 200ul
EUR 552
Description: Pre-made lentivirus express GFP reporter under a minimal promoter that embedded 8 tandem repeats of AP1 Responsive Element (AP1-RE) . This lentivirus also contain the Neomycin selection marker under RSV promoter, provided in DMEM medium with 10% FBS and 60ug/ml of polybrene.
AP1-GFP (Puro) Lentivirus 
LVP953-P 1x107 IFU/ml x 200ul
EUR 552
Description: Pre-made lentivirus express GFP reporter under a minimal promoter that embedded 8 tandem repeats of AP1 Responsive Element (AP1-RE) . This lentivirus also contain the puromycin selection marker under RSV promoter, provided in DMEM medium with 10% FBS and 60ug/ml of polybrene.
AP1-GFP (RFP) Lentivirus 
LVP953-R 1x107 IFU/ml x 200ul
EUR 552
Description: Pre-made lentivirus express GFP reporter under a minimal promoter that embedded 8 tandem repeats of AP1 Responsive Element (AP1-RE) . This lentivirus also contain the RFP selection marker under the constitutive RSV promoter, provided in DMEM medium with 10% FBS and 60ug/ml of polybrene.
AP1-RFP (GFP) Lentivirus 
LVP954-G 1x107 IFU/ml x 200ul
EUR 552
Description: Pre-made lentivirus express RFP reporter under a minimal promoter that embedded 8 tandem repeats of AP1 Responsive Element (AP1-RE) . This lentivirus also contain the GFP selection marker under the consitutive RSV promoter, provided in DMEM medium with 10% FBS and 60ug/ml of polybrene.
AP1-Luc (GFP) Lentivirus 
LVP955-G 1x107 IFU/ml x 200ul
EUR 552
Description: Pre-made lentivirus express Firefly luciferase reporter under a minimal promoter that embedded 8 tandem repeats of AP1 Responsive Element (AP1-RE) . This lentivirus also contain the GFP selection marker under the consitutiveRSV promoter, provided in DMEM medium with 10% FBS and 60ug/ml of polybrene.
AP1-Rluc (GFP) Lentivirus 
LVP956-G 1x107 IFU/ml x 200ul
EUR 552
Description: Pre-made lentivirus express Renilla luciferase reporter under a minimal promoter that embedded 8 tandem repeats of AP1 Responsive Element (AP1-RE) . This lentivirus also contain the GFP selection marker under the consitutive RSV promoter, provided in DMEM medium with 10% FBS and 60ug/ml of polybrene.
SRE-GFP (Bsd) Lentivirus 
LVP957-B 1x107 IFU/ml x 200ul
EUR 552
Description: Pre-made lentivirus express GFP reporter under a minimal promoter that embedded 4 tandem repeats of serum response element (SRE) . This lentivirus also contain the Blasticidin selection marker under RSV promoter, provided in DMEM medium with 10% FBS and 60ug/ml of polybrene.
SRE-GFP (Neo) Lentivirus 
LVP957-N 1x107 IFU/ml x 200ul
EUR 552
Description: Pre-made lentivirus express GFP reporter under a minimal promoter that embedded 4 tandem repeats of serum response element (SRE) . This lentivirus also contain the Neomycin selection marker under RSV promoter, provided in DMEM medium with 10% FBS and 60ug/ml of polybrene.
SRE-GFP (Puro) Lentivirus 
LVP957-P 1x107 IFU/ml x 200ul
EUR 552
Description: Pre-made lentivirus express GFP reporter under a minimal promoter that embedded 4 tandem repeats of serum response element (SRE) . This lentivirus also contain the puromycin selection marker under RSV promoter, provided in DMEM medium with 10% FBS and 60ug/ml of polybrene.
SRE-GFP (RFP) Lentivirus 
LVP957-R 1x107 IFU/ml x 200ul
EUR 552
Description: Pre-made lentivirus express GFP reporter under a minimal promoter that embedded 4 tandem repeats of serum response element (SRE) . This lentivirus also contain the RFP selection marker under the constitutive RSV promoter, provided in DMEM medium with 10% FBS and 60ug/ml of polybrene.
SRE-RFP (GFP) Lentivirus 
LVP958-G 1x107 IFU/ml x 200ul
EUR 552
Description: Pre-made lentivirus express RFP reporter under a minimal promoter that embedded 4 tandem repeats of serum response element (SRE) . This lentivirus also contain the GFP selection marker under the consitutive RSV promoter, provided in DMEM medium with 10% FBS and 60ug/ml of polybrene.
SRE-Luc (GFP) Lentivirus 
LVP959-G 1x107 IFU/ml x 200ul
EUR 552
Description: Pre-made lentivirus express Firefly luciferase reporter under a minimal promoter that embedded 4 tandem repeats of serum response element (SRE) . This lentivirus also contain the GFP selection marker under the consitutiveRSV promoter, provided in DMEM medium with 10% FBS and 60ug/ml of polybrene.
SRE-Rluc (GFP) Lentivirus 
LVP960-G 1x107 IFU/ml x 200ul
EUR 552
Description: Pre-made lentivirus express Renilla luciferase reporter under a minimal promoter that embedded 4 tandem repeats of serum response element (SRE) . This lentivirus also contain the GFP selection marker under the consitutive RSV promoter, provided in DMEM medium with 10% FBS and 60ug/ml of polybrene.
RBP_JK-GFP (Bsd) Lentivirus 
LVP961-B 1x107 IFU/ml x 200ul
EUR 552
Description: Pre-made lentivirus express GFP reporter under a minimal promoter that embedded 4 tandem repeats of RBP-JK transcriptional responsive element (RBP-JK TRE). This lentivirus also contain the Blasticidin selection marker under RSV promoter, provided in DMEM medium with 10% FBS and 60ug/ml of polybrene.
RBP_JK-GFP (Neo) Lentivirus 
LVP961-N 1x107 IFU/ml x 200ul
EUR 552
Description: Pre-made lentivirus express GFP reporter under a minimal promoter that embedded 4 tandem repeats of RBP-JK transcriptional responsive element (RBP-JK TRE). This lentivirus also contain the Neomycin selection marker under RSV promoter, provided in DMEM medium with 10% FBS and 60ug/ml of polybrene.
RBP_JK-GFP (Puro) Lentivirus 
LVP961-P 1x107 IFU/ml x 200ul
EUR 552
Description: Pre-made lentivirus express GFP reporter under a minimal promoter that embedded 4 tandem repeats of RBP-JK transcriptional responsive element (RBP-JK TRE). This lentivirus also contain the puromycin selection marker under RSV promoter, provided in DMEM medium with 10% FBS and 60ug/ml of polybrene.
RBP_JK-GFP (RFP) Lentivirus 
LVP961-R 1x107 IFU/ml x 200ul
EUR 552
Description: Pre-made lentivirus express GFP reporter under a minimal promoter that embedded 4 tandem repeats of RBP-JK transcriptional responsive element (RBP-JK TRE). This lentivirus also contain the RFP selection marker under the constitutive RSV promoter, provided in DMEM medium with 10% FBS and 60ug/ml of polybrene.
RBP_JK-RFP (GFP) Lentivirus 
LVP962-G 1x107 IFU/ml x 200ul
EUR 552
Description: Pre-made lentivirus express RFP reporter under a minimal promoter that embedded 4 tandem repeats of RBP-JK transcriptional responsive element (RBP-JK TRE). This lentivirus also contain the GFP selection marker under the consitutive RSV promoter, provided in DMEM medium with 10% FBS and 60ug/ml of polybrene.
RBP_JK-Luc (GFP) Lentivirus 
LVP963-G 1x107 IFU/ml x 200ul
EUR 552
Description: Pre-made lentivirus express Firefly luciferase reporter under a minimal promoter that embedded 4 tandem repeats of RBP-JK transcriptional responsive element (RBP-JK TRE). This lentivirus also contain the GFP selection marker under the consitutiveRSV promoter, provided in DMEM medium with 10% FBS and 60ug/ml of polybrene.
RBP_JK-Rluc (GFP) Lentivirus 
LVP964-G 1x107 IFU/ml x 200ul
EUR 552
Description: Pre-made lentivirus express Renilla luciferase reporter under a minimal promoter that embedded 4 tandem repeats of RBP-JK transcriptional responsive element (RBP-JK TRE). This lentivirus also contain the GFP selection marker under the consitutive RSV promoter, provided in DMEM medium with 10% FBS and 60ug/ml of polybrene.
NFkB-GFP (Bsd) Lentivirus 
LVP965-B 1x107 IFU/ml x 200ul
EUR 552
Description: Pre-made lentivirus express GFP reporter under a minimal promoter that embedded 4 tandem repeats of NFκB transcriptional response element (NFkB-TRE). This lentivirus also contain the Blasticidin selection marker under RSV promoter, provided in DMEM medium with 10% FBS and 60ug/ml of polybrene.
NFkB-GFP (Neo) Lentivirus 
LVP965-N 1x107 IFU/ml x 200ul
EUR 552
Description: Pre-made lentivirus express GFP reporter under a minimal promoter that embedded 4 tandem repeats of NFκB transcriptional response element (NFkB-TRE). This lentivirus also contain the Neomycin selection marker under RSV promoter, provided in DMEM medium with 10% FBS and 60ug/ml of polybrene.
NFkB-GFP (Puro) Lentivirus 
LVP965-P 1x107 IFU/ml x 200ul
EUR 552
Description: Pre-made lentivirus express GFP reporter under a minimal promoter that embedded 4 tandem repeats of NFκB transcriptional response element (NFkB-TRE). This lentivirus also contain the puromycin selection marker under RSV promoter, provided in DMEM medium with 10% FBS and 60ug/ml of polybrene.
NFkB-GFP (RFP) Lentivirus 
LVP965-R 1x107 IFU/ml x 200ul
EUR 552
Description: Pre-made lentivirus express GFP reporter under a minimal promoter that embedded 4 tandem repeats of NFκB transcriptional response element (NFkB-TRE). This lentivirus also contain the RFP selection marker under the constitutive RSV promoter, provided in DMEM medium with 10% FBS and 60ug/ml of polybrene.
NFkB-RFP (GFP) Lentivirus 
LVP966-G 1x107 IFU/ml x 200ul
EUR 552
Description: Pre-made lentivirus express RFP reporter under a minimal promoter that embedded 4 tandem repeats of NFκB transcriptional response element (NFkB-TRE). This lentivirus also contain the GFP selection marker under the consitutive RSV promoter, provided in DMEM medium with 10% FBS and 60ug/ml of polybrene.
NFkB-Luc (GFP) Lentivirus 
LVP967-G 1x107 IFU/ml x 200ul
EUR 552
Description: Pre-made lentivirus express Firefly luciferase reporter under a minimal promoter that embedded 4 tandem repeats of NFκB transcriptional response element (NFkB-TRE). This lentivirus also contain the GFP selection marker under the consitutiveRSV promoter, provided in DMEM medium with 10% FBS and 60ug/ml of polybrene.
NFkB-Rluc (GFP) Lentivirus 
LVP968-G 1x107 IFU/ml x 200ul
EUR 552
Description: Pre-made lentivirus express Renilla luciferase reporter under a minimal promoter that embedded 4 tandem repeats of NFκB transcriptional response element (NFkB-TRE). This lentivirus also contain the GFP selection marker under the consitutive RSV promoter, provided in DMEM medium with 10% FBS and 60ug/ml of polybrene.
H_RE-GFP (Bsd) Lentivirus 
LVP969-B 1x107 IFU/ml x 200ul
EUR 552
Description: Pre-made lentivirus express GFP reporter under a minimal promoter that embedded 6 tandem repeats of hypoxia transcriptional response element (H-RE). This lentivirus also contain the Blasticidin selection marker under RSV promoter, provided in DMEM medium with 10% FBS and 60ug/ml of polybrene.
H_RE-GFP (Neo) Lentivirus 
LVP969-N 1x107 IFU/ml x 200ul
EUR 552
Description: Pre-made lentivirus express GFP reporter under a minimal promoter that embedded 6 tandem repeats of hypoxia transcriptional response element (H-RE). This lentivirus also contain the Neomycin selection marker under RSV promoter, provided in DMEM medium with 10% FBS and 60ug/ml of polybrene.
H_RE-GFP (Puro) Lentivirus 
LVP969-P 1x107 IFU/ml x 200ul
EUR 552
Description: Pre-made lentivirus express GFP reporter under a minimal promoter that embedded 6 tandem repeats of hypoxia transcriptional response element (H-RE). This lentivirus also contain the puromycin selection marker under RSV promoter, provided in DMEM medium with 10% FBS and 60ug/ml of polybrene.
H_RE-GFP (RFP) Lentivirus 
LVP969-R 1x107 IFU/ml x 200ul
EUR 552
Description: Pre-made lentivirus express GFP reporter under a minimal promoter that embedded 6 tandem repeats of hypoxia transcriptional response element (H-RE). This lentivirus also contain the RFP selection marker under the constitutive RSV promoter, provided in DMEM medium with 10% FBS and 60ug/ml of polybrene.
H_RE-RFP (GFP) Lentivirus 
LVP970-G 1x107 IFU/ml x 200ul
EUR 552
Description: Pre-made lentivirus express RFP reporter under a minimal promoter that embedded 6 tandem repeats of hypoxia transcriptional response element (H-RE). This lentivirus also contain the GFP selection marker under the consitutive RSV promoter, provided in DMEM medium with 10% FBS and 60ug/ml of polybrene.
H_RE-Luc (GFP) Lentivirus 
LVP971-G 1x107 IFU/ml x 200ul
EUR 552
Description: Pre-made lentivirus express Firefly luciferase reporter under a minimal promoter that embedded 6 tandem repeats of hypoxia transcriptional response element (H-RE). This lentivirus also contain the GFP selection marker under the consitutiveRSV promoter, provided in DMEM medium with 10% FBS and 60ug/ml of polybrene.
H_RE-Rluc (GFP) Lentivirus 
LVP972-G 1x107 IFU/ml x 200ul
EUR 552
Description: Pre-made lentivirus express Renilla luciferase reporter under a minimal promoter that embedded 6 tandem repeats of hypoxia transcriptional response element (H-RE). This lentivirus also contain the GFP selection marker under the consitutive RSV promoter, provided in DMEM medium with 10% FBS and 60ug/ml of polybrene.
ER-GFP (Bsd) Lentivirus 
LVP973-B 1x107 IFU/ml x 200ul
EUR 552
Description: Pre-made lentivirus express GFP reporter under a minimal promoter that embedded 4 tandem repeats of estrogen receptor response element (ER-RE). This lentivirus also contain the Blasticidin selection marker under RSV promoter, provided in DMEM medium with 10% FBS and 60ug/ml of polybrene.
ER-GFP (Neo) Lentivirus 
LVP973-N 1x107 IFU/ml x 200ul
EUR 552
Description: Pre-made lentivirus express GFP reporter under a minimal promoter that embedded 4 tandem repeats of estrogen receptor response element (ER-RE). This lentivirus also contain the Neomycin selection marker under RSV promoter, provided in DMEM medium with 10% FBS and 60ug/ml of polybrene.
ER-GFP (Puro) Lentivirus 
LVP973-P 1x107 IFU/ml x 200ul
EUR 552
Description: Pre-made lentivirus express GFP reporter under a minimal promoter that embedded 4 tandem repeats of estrogen receptor response element (ER-RE). This lentivirus also contain the puromycin selection marker under RSV promoter, provided in DMEM medium with 10% FBS and 60ug/ml of polybrene.
ER-GFP (RFP) Lentivirus 
LVP973-R 1x107 IFU/ml x 200ul
EUR 552
Description: Pre-made lentivirus express GFP reporter under a minimal promoter that embedded 4 tandem repeats of estrogen receptor response element (ER-RE). This lentivirus also contain the RFP selection marker under the constitutive RSV promoter, provided in DMEM medium with 10% FBS and 60ug/ml of polybrene.
ER-RFP (GFP) Lentivirus 
LVP974-G 1x107 IFU/ml x 200ul
EUR 552
Description: Pre-made lentivirus express RFP reporter under a minimal promoter that embedded 4 tandem repeats of estrogen receptor response element (ER-RE). This lentivirus also contain the GFP selection marker under the consitutive RSV promoter, provided in DMEM medium with 10% FBS and 60ug/ml of polybrene.
ER-Luc (GFP) Lentivirus 
LVP975-G 1x107 IFU/ml x 200ul
EUR 552
Description: Pre-made lentivirus express Firefly luciferase reporter under a minimal promoter that embedded 4 tandem repeats of estrogen receptor response element (ER-RE). This lentivirus also contain the GFP selection marker under the consitutiveRSV promoter, provided in DMEM medium with 10% FBS and 60ug/ml of polybrene.
ER-Rluc (GFP) Lentivirus 
LVP976-G 1x107 IFU/ml x 200ul
EUR 552
Description: Pre-made lentivirus express Renilla luciferase reporter under a minimal promoter that embedded 4 tandem repeats of estrogen receptor response element (ER-RE). This lentivirus also contain the GFP selection marker under the consitutive RSV promoter, provided in DMEM medium with 10% FBS and 60ug/ml of polybrene.
P53-GFP (Bsd) Lentivirus 
LVP977-B 1x107 IFU/ml x 200ul
EUR 552
Description: Pre-made lentivirus express GFP reporter under a minimal promoter that embedded with optimized tandem repeats of a few most potent P53 transcriptional response element. This lentivirus also contain the Blasticidin selection marker under RSV promoter, provided in DMEM medium with 10% FBS and 60ug/ml of polybrene.
P53-GFP (Neo) Lentivirus 
LVP977-N 1x107 IFU/ml x 200ul
EUR 552
Description: Pre-made lentivirus express GFP reporter under a minimal promoter that embedded with optimized tandem repeats of a few most potent P53 transcriptional response element. This lentivirus also contain the Neomycin selection marker under RSV promoter, provided in DMEM medium with 10% FBS and 60ug/ml of polybrene.
P53-GFP (Puro) Lentivirus 
LVP977-P 1x107 IFU/ml x 200ul
EUR 552
Description: Pre-made lentivirus express GFP reporter under a minimal promoter that embedded with optimized tandem repeats of a few most potent P53 transcriptional response element. This lentivirus also contain the puromycin selection marker under RSV promoter, provided in DMEM medium with 10% FBS and 60ug/ml of polybrene.
P53-GFP (RFP) Lentivirus 
LVP977-R 1x107 IFU/ml x 200ul
EUR 552
Description: Pre-made lentivirus express GFP reporter under a minimal promoter that embedded with optimized tandem repeats of a few most potent P53 transcriptional response element. This lentivirus also contain the RFP selection marker under the constitutive RSV promoter, provided in DMEM medium with 10% FBS and 60ug/ml of polybrene.
P53-RFP (GFP) Lentivirus 
LVP978-G 1x107 IFU/ml x 200ul
EUR 552
Description: Pre-made lentivirus express RFP reporter under a minimal promoter that embedded with optimized tandem repeats of a few most potent P53 transcriptional response element. This lentivirus also contain the GFP selection marker under the consitutive RSV promoter, provided in DMEM medium with 10% FBS and 60ug/ml of polybrene.
P53-Luc (GFP) Lentivirus 
LVP979-G 1x107 IFU/ml x 200ul
EUR 552
Description: Pre-made lentivirus express Firefly luciferase reporter under a minimal promoter that embedded with optimized tandem repeats of a few most potent P53 transcriptional response element. This lentivirus also contain the GFP selection marker under the consitutiveRSV promoter, provided in DMEM medium with 10% FBS and 60ug/ml of polybrene.
P53-Rluc (GFP) Lentivirus 
LVP980-G 1x107 IFU/ml x 200ul
EUR 552
Description: Pre-made lentivirus express Renilla luciferase reporter under a minimal promoter that embedded with optimized tandem repeats of a few most potent P53 transcriptional response element. This lentivirus also contain the GFP selection marker under the consitutive RSV promoter, provided in DMEM medium with 10% FBS and 60ug/ml of polybrene.
ARE-GFP (Bsd) Lentivirus 
LVP981-B 1x107 IFU/ml x 200ul
EUR 552
Description: Pre-made lentivirus express GFP reporter under a minimal promoter that embedded 4 tandem repeats of antioxidant response element (ARE). This lentivirus also contain the Blasticidin selection marker under RSV promoter, provided in DMEM medium with 10% FBS and 60ug/ml of polybrene.
ARE-GFP (Neo) Lentivirus 
LVP981-N 1x107 IFU/ml x 200ul
EUR 552
Description: Pre-made lentivirus express GFP reporter under a minimal promoter that embedded 4 tandem repeats of antioxidant response element (ARE). This lentivirus also contain the Neomycin selection marker under RSV promoter, provided in DMEM medium with 10% FBS and 60ug/ml of polybrene.
ARE-GFP (Puro) Lentivirus
LVP981-P 1x107 IFU/ml x 200ul
EUR 552
Description: Pre-made lentivirus express GFP reporter under a minimal promoter that embedded 4 tandem repeats of antioxidant response element (ARE). This lentivirus also contain the puromycin selection marker under RSV promoter, provided in DMEM medium with 10% FBS and 60ug/ml of polybrene.
ARE-GFP (RFP) Lentivirus 
LVP981-R 1x107 IFU/ml x 200ul
EUR 552
Description: Pre-made lentivirus express GFP reporter under a minimal promoter that embedded 4 tandem repeats of antioxidant response element (ARE). This lentivirus also contain the RFP selection marker under the constitutive RSV promoter, provided in DMEM medium with 10% FBS and 60ug/ml of polybrene.
ARE-RFP (GFP) Lentivirus 
LVP982-G 1x107 IFU/ml x 200ul
EUR 552
Description: Pre-made lentivirus express RFP reporter under a minimal promoter that embedded 4 tandem repeats of antioxidant response element (ARE). This lentivirus also contain the GFP selection marker under the consitutive RSV promoter, provided in DMEM medium with 10% FBS and 60ug/ml of polybrene.
ARE-Luc (GFP) Lentivirus 
LVP983-G 1x107 IFU/ml x 200ul
EUR 552
Description: Pre-made lentivirus express Firefly luciferase reporter under a minimal promoter that embedded 4 tandem repeats of antioxidant response element (ARE). This lentivirus also contain the GFP selection marker under the consitutiveRSV promoter, provided in DMEM medium with 10% FBS and 60ug/ml of polybrene.
ARE-Rluc (GFP) Lentivirus 
LVP984-G 1x107 IFU/ml x 200ul
EUR 552
Description: Pre-made lentivirus express Renilla luciferase reporter under a minimal promoter that embedded 4 tandem repeats of antioxidant response element (ARE). This lentivirus also contain the GFP selection marker under the consitutive RSV promoter, provided in DMEM medium with 10% FBS and 60ug/ml of polybrene.
CEBP-GFP (Bsd) Lentivirus 
LVP985-B 1x107 IFU/ml x 200ul
EUR 552
Description: Pre-made lentivirus express GFP reporter under a minimal promoter that embedded 8 tandem repeats of C/EBP transcriptional response element (C/EBP-TRE). This lentivirus also contain the Blasticidin selection marker under RSV promoter, provided in DMEM medium with 10% FBS and 60ug/ml of polybrene.
CEBP-GFP (Neo) Lentivirus 
LVP985-N 1x107 IFU/ml x 200ul
EUR 552
Description: Pre-made lentivirus express GFP reporter under a minimal promoter that embedded 8 tandem repeats of C/EBP transcriptional response element (C/EBP-TRE). This lentivirus also contain the Neomycin selection marker under RSV promoter, provided in DMEM medium with 10% FBS and 60ug/ml of polybrene.

Self-Organizing Human Induced Pluripotent Stem Cell Hepatocyte 3D Organoids Inform the Biology of the Pleiotropic TRIB1 Gene

Institution of a physiologically related human hepatocyte-like cell system for in vitro translational analysis has been hampered by the restricted availability of cell fashions that precisely replicate human biology and the pathophysiology of human illness. Right here we report a strong, reproducible, and scalable protocol for the technology of hepatic organoids from human induced pluripotent stem cells (hiPSCs) utilizing brief publicity to nonengineered matrices.

These hepatic organoids observe outlined levels of hepatic improvement and categorical greater ranges of early (hepatocyte nuclear issue 4A [HNF4A], prospero-related homeobox 1 [PROX1]) and mature hepatic and metabolic markers (albumin, asialoglycoprotein receptor 1 [ASGR1], CCAAT/enhancer binding protein α [C/EBPα]) than two-dimensional (2D) hepatocyte-like cells (HLCs) at day 20 of differentiation.

We used this mannequin to discover the biology of the pleiotropic TRIB1 (Tribbles-1) gene related to a variety of metabolic traits, together with nonalcoholic fatty liver illness and plasma lipids.

We used genome enhancing to delete the TRIB1 gene in hiPSCs and in contrast TRIB1-deleted iPSC-HLCs to isogenic iPSC-HLCs underneath each 2D tradition and three-dimensional (3D) organoid circumstances. Beneath standard 2D tradition circumstances, TRIB1-deficient HLCs confirmed maturation defects, with decreased expression of late-stage hepatic and lipogenesis markers.

In distinction, when cultured as 3D hepatic organoids, the differentiation defects have been rescued, and a transparent lipid-related phenotype was famous within the TRIB1-deficient induced pluripotent stem cell HLCs. Conclusion: This work helps the potential of genome-edited hiPSC-derived hepatic 3D organoids in exploring human hepatocyte biology, together with the practical interrogation of genes recognized by means of human genetic investigation.

MCF-7 Nuclear Extract

X12002 1000 µg Ask for price
Description: The best epigenetics products

Genomic DNA from Human Tumor Cell Line: MCF 7

D1255830 100 ug
EUR 243
Description: Can be used for various studies in the realm of gene expression, both normal and pathological. It is an excellent control and suitable for educational purposes.

Membrane Protein from Human Tumor Cell Line: MCF 7

P3255830 0.1 mg
EUR 285
Description: Can be used for various proteomics studies in both normal and pathological cases. It is an excellent control and suitable for educational purposes. This product is prepared from whole tissue homogenates and has undergone SDS-PAGE quality control analysis. The protein is stored in a buffer with protease inhibitor cocktail fo prevent degradation.

Total Protein from Human Tumor Cell Line: MCF 7

P1255830 1 mg
EUR 214
Description: Can be used for various proteomics studies in both normal and pathological cases. It is an excellent control and suitable for educational purposes. This product is prepared from whole tissue homogenates and has undergone SDS-PAGE quality control analysis. The protein is stored in a buffer with protease inhibitor cocktail fo prevent degradation.

Total RNA from Human Tumor Cell Line: MCF 7

R1255830-50 50 ug
EUR 194
Description: Can be used for various studies in the realm of gene expression and regulation, both normal and pathological. It is an excellent control and suitable for educational purposes.

Paraffin Tissue Section - Human Tumor Cell Line: MCF-7

T2255830 5 slides
EUR 257
Description: Our tissue products are produced by strictly following the IRB ethical standards and procedures and from highest quality tissues. Immediately after collection the tissues are placed in liquid nitrogen and examined by certified pathologists. The thickness of each individual section is ~5um. They are Hematoxylin and Eosin stained and quality tested by immunostaining with anti-beta-actin antibodies. Our tissue products are suitable for various studies on cellular level (RNA localization, Protein expression, etc.) on both normal and pathological cases. It is also an excellent control and educational tool.

MCF-7 Nuclear Extract (H2O2)

1642-100
EUR 207

Human MCF-7 (breast cancer) Cell Nuclear Extract

HCL-2016 100ug
EUR 213

293/GFP Cell Line

AKR-200 1 vial
EUR 572
Description: 293/GFP Cell Line stably expresses GFP and otherwise exhibits the same characteristics of the parental cell line.

T47D/GFP Cell Line

AKR-208 1 vial
EUR 572
Description: T47D/GFP Cell Line stably expresses GFP and otherwise exhibits the same characteristics of the parental cell line.

A549/GFP Cell Line

AKR-209 1 vial
EUR 572
Description: A549/GFP Cell Line stably expresses GFP and otherwise exhibits the same characteristics of the parental cell line.

HeLa/GFP Cell Line

AKR-213 1 vial
EUR 572
Description: HeLa/GFP Cell Line stably expresses GFP and otherwise exhibits the same characteristics of the parental cell line.

NIH3T3/GFP Cell Line

AKR-214 1 vial
EUR 572
Description: NIH3T3/GFP Cell Line stably expresses GFP and otherwise exhibits the same characteristics of the parental cell line.

MCF-7 Whole Cell Lysate (Human breast adenocarcinoma cells)

MCF7-100 100 ug
EUR 164

MCF-7 Whole Cell Lysate (Human breast adenocarcinoma cells)

MCF7-50 50 ug
EUR 128

MCF-7 Human breast cancer, noninvasive cell line: >1x10^10 frozen exosomes

EXOP-100A-1 50 ug
EUR 467
  • Category: Exosomes

MCF-10A

C0006015 One Frozen vial
EUR 467

Human MCF-7 (breast cancer) Whole Cell Lysate, Hydrogen Peroxide Stimulated

HCL-2014 100ug
EUR 213

GFP Expressing Human Glioblastoma Cells

TR01-GFP 500,000 Cells
EUR 1354

GFP Expressing Human Gastric Carcinoma N87 Cells

TR02-GFP 500,000 Cells
EUR 1354

GFP Expressing Human Renal Adenocarcinoma Cells (ACHN)

TR04-GFP 500,000 Cells
EUR 1354

GFP Expressing Human Prostate Carcinoma Cells (DU 145)

TR03-GFP 500,000 Cells
EUR 1354

293AD Cell Line

AD-100 1 vial
EUR 461
Description: The 293AD Cell Line is derived from the parental 293 cells but selected for attributes that increase adenovirus production, including firmer attachment and larger surface area.

293AAV Cell Line

AAV-100 1 vial
EUR 508
Description: The 293AAV Cell Line is derived from the parental 293 cells but selected for attributes that increase AAV production, including firmer attachment and larger surface area.

293LTV Cell Line

LTV-100 1 vial
EUR 508
Description: The 293LTV Cell Line is derived from the parental 293 cells but selected for attributes that increase lentiviral production, including fimrer attachment and larger surface area.

293RTV Cell Line

RV-100 1 vial
EUR 508
Description: The 293RTV Cell Line is derived from the parental 293 cells but selected for attributes that increase retroviral production, including fimrer attachment and larger surface area.

NIH3T3/Cas9 Cell Line

AKR-5104 1 vial
EUR 572

293/Cas9 Cell Line

AKR-5110 1 vial
EUR 572

HeLa/Cas9 Cell Line

AKR-5111 1 vial
EUR 572

Krtap16-7 3'UTR GFP Stable Cell Line

TU160815 1.0 ml Ask for price

Krtap4-7 3'UTR GFP Stable Cell Line

TU160834 1.0 ml Ask for price

Krtap4-7 3'UTR GFP Stable Cell Line

TU256900 1.0 ml Ask for price

IGHD1-7 3'UTR GFP Stable Cell Line

TU060550 1.0 ml
EUR 2333

IGHV3-7 3'UTR GFP Stable Cell Line

TU060641 1.0 ml
EUR 2333

IGHV3OR15-7 3'UTR GFP Stable Cell Line

TU060691 1.0 ml
EUR 2333

IGHV3OR16-7 3'UTR GFP Stable Cell Line

TU060693 1.0 ml
EUR 2333

IGKV2OR2-7 3'UTR GFP Stable Cell Line

TU060855 1.0 ml
EUR 2333

IGKV3-7 3'UTR GFP Stable Cell Line

TU060861 1.0 ml
EUR 2333

IGKV3D-7 3'UTR GFP Stable Cell Line

TU060868 1.0 ml
EUR 2333

IGLV3-7 3'UTR GFP Stable Cell Line

TU060934 1.0 ml
EUR 2333

KRTAP4-7 3'UTR GFP Stable Cell Line

TU062120 1.0 ml
EUR 1394

KRTAP5-7 3'UTR GFP Stable Cell Line

TU062132 1.0 ml
EUR 1394

KRTAP9-7 3'UTR GFP Stable Cell Line

TU062151 1.0 ml
EUR 1394

KRTAP10-7 3'UTR GFP Stable Cell Line

TU062161 1.0 ml
EUR 1394

KRTAP19-7 3'UTR GFP Stable Cell Line

TU062187 1.0 ml
EUR 1394

TRAV8-7 3'UTR GFP Stable Cell Line

TU076322 1.0 ml
EUR 2333

TRBJ2-7 3'UTR GFP Stable Cell Line

TU076380 1.0 ml
EUR 2333

TRBV5-7 3'UTR GFP Stable Cell Line

TU076394 1.0 ml
EUR 2333

TRBV6-7 3'UTR GFP Stable Cell Line

TU076402 1.0 ml
EUR 2333

TRBV7-7 3'UTR GFP Stable Cell Line

TU076411 1.0 ml
EUR 2333

RNU6-7 3'UTR GFP Stable Cell Line

TU070174 1.0 ml
EUR 2333

SKOV-3/Luc Cell Line

AKR-232 1 vial
EUR 572
Description: SKOV-3/Luc Cell Line stably expresses luciferase and otherwise exhibits the same characteristics of the parental cell line.

OVCAR-5/RFP Cell Line

AKR-254 1 vial
EUR 572
Description: OVCAR-5/RFP Cell Line stably expresses RFP and otherwise exhibits the same characteristics of the parental cell line.

Platinum-E Retroviral Packaging Cell Line, Ecotropic

RV-101 1 vial
EUR 920
Description: Conventional cells used for retrovirus packaging, such as those based on NIH3T3 cells, have limited stability and produce relatively low yields of retrovirus, mainly due to the poor expression of retroviral structure proteins (gag, pol and env) in the cells. The Platinum Retroviral Packaging Cell Lines are based on the 293T cell line. They exhibit longer stability and produce higher yields of retroviral structure proteins. Plat-E cells contain gag, pol and env genes, allowing retroviral packaging with a single plasmid transfection.

Platinum-A Retroviral Packaging Cell Line, Amphotropic

RV-102 1 vial
EUR 920
Description: Conventional cells used for retrovirus packaging, such as those based on NIH3T3 cells, have limited stability and produce relatively low yields of retrovirus, mainly due to the poor expression of retroviral structure proteins (gag, pol and env) in the cells. The Platinum Retroviral Packaging Cell Lines are based on the 293T cell line. They exhibit longer stability and produce higher yields of retroviral structure proteins. Plat-A cells contain gag, pol and env genes, allowing retroviral packaging with a single plasmid transfection.

Platinum-GP Retroviral Packaging Cell Line, Pantropic

RV-103 1 vial
EUR 920
Description: Conventional cells used for retrovirus packaging, such as those based on NIH3T3 cells, have limited stability and produce relatively low yields of retrovirus, mainly due to the poor expression of retroviral structure proteins (gag, pol and env) in the cells. The Platinum Retroviral Packaging Cell Lines are based on the 293T cell line. They exhibit longer stability and produce higher yields of retroviral structure proteins. Plat-GP cells contain the gag and pol genes required for retroviral packaging; an expression vector is co-transfected with a VSVG envelope vector.

Green Fluorescent Protein (GFP-fusion protein) ELISA Kit, 96 tests, Quantitative

800-420-GFP 1 kit
EUR 712

Total Protein - Murine Embryonic Stem Cell Line D3

CBA-305 500 ?g
EUR 345
Description:
  • Isolated from mouse ES-D3 cell line
  • Presented as 500 µg at 1 mg/mL in NP-40 Solubilization Buffer

Anti-Cytokeratin 7

DB051RTU-7 7 ml
EUR 204
Description: rabbit monospecific clonal antibodies for ihc-p application; prediluted (ready to use)

Recombinant Human Galectin-7

7-00442 2µg Ask for price

Recombinant Human Galectin-7

7-00443 10µg Ask for price

Recombinant Human Galectin-7

7-00444 1mg Ask for price

Recombinant Human Interleukin-7

7-00895 2µg Ask for price

Recombinant Human Interleukin-7

7-00896 10µg Ask for price

Recombinant Human Interleukin-7

7-00897 1mg Ask for price

Recombinant Mouse Interleukin-7

7-00904 2µg Ask for price

Recombinant Mouse Interleukin-7

7-00905 10µg Ask for price

Recombinant Mouse Interleukin-7

7-00906 1mg Ask for price

Recombinant Human Kallikrein-7

7-03034 2µg Ask for price

Recombinant Human Kallikrein-7

7-03035 10µg Ask for price

Recombinant Human Kallikrein-7

7-03036 100µg Ask for price

Recombinant ProMatrix Metalloproteinase-7

7-03478 5µg Ask for price

Recombinant ProMatrix Metalloproteinase-7

7-03479 20µg Ask for price

Recombinant ProMatrix Metalloproteinase-7

7-03480 1mg Ask for price

Individual Reaction Mix 7

G065-7 200 reactions
EUR 167

Recombinant Human Interleukin-7, Saccharomyces

7-00898 2µg Ask for price

Recombinant Human Interleukin-7, Saccharomyces

7-00899 10µg Ask for price

Recombinant Human Interleukin-7, Saccharomyces

7-00900 1mg Ask for price

Recombinant Human Matrix Metalloproteinase-7

7-03154 5µg Ask for price

Recombinant Human Matrix Metalloproteinase-7

7-03155 20µg Ask for price

Recombinant Human Matrix Metalloproteinase-7

7-03156 1mg Ask for price

Human macrophage chemotatic factor,MCF ELISA Kit

201-12-0070 96 tests
EUR 440
  • This macrophage chemotatic factor ELISA kit is validated to work with samples from whole blood, serum, plasma and cell culture supernatant.
Description: A quantitative ELISA kit for measuring Human in samples from biological fluids.

Human macrophage chemotatic factor,MCF ELISA Kit

CN-04498H1 96T
EUR 452

Human macrophage chemotatic factor,MCF ELISA Kit

CN-04498H2 48T
EUR 302

Monkey Macrophage chemotactic Factor (MCF) ELISA Kit

abx359979-96tests 96 tests
EUR 825
  • Shipped within 5-12 working days.

Pig Macrophage chemotactic Factor (MCF) ELISA Kit

abx361774-96tests 96 tests
EUR 825
  • Shipped within 5-12 working days.

Rabbit Macrophage chemotactic Factor (MCF) ELISA Kit

abx362676-96tests 96 tests
EUR 825
  • Shipped within 5-12 working days.

Human Macrophage Chemotactic Factor (MCF) ELISA Kit

abx354411-96tests 96 tests
EUR 786
  • Shipped within 5-12 working days.

Chicken Macrophage chemotactic Factor (MCF) ELISA Kit

abx356572-96tests 96 tests
EUR 825
  • Shipped within 5-12 working days.

Canine macrophage chemotatic factor(MCF)ELISA Kit

GA-E0087CN-48T 48T
EUR 402

Canine macrophage chemotatic factor(MCF)ELISA Kit

GA-E0087CN-96T 96T
EUR 684

Human macrophage chemotatic factor(MCF)ELISA Kit

GA-E0109HM-48T 48T
EUR 289

Human macrophage chemotatic factor(MCF)ELISA Kit

GA-E0109HM-96T 96T
EUR 466

Human macrophage chemotatic factor(MCF)ELISA Kit

QY-E02720 96T
EUR 361

GFP Recombinant Adenovirus

ADV-004 50 ?L
EUR 891
Description: Premade recombinant adenovirus containing the GFP gene

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