N-Glycosylation Modification of Butyrylcholinesterase in a Transgenic Rice CellCulture

N-Glycosylation Modification of Butyrylcholinesterase in a Transgenic Rice CellCulture

Propofol Impacts Non-Small-Cell Lung Most cancers Cell Biology By Regulating the miR-21/PTEN/AKT Pathway In Vitro and In Vivo 

Background: Propofol is a typical sedative-hypnotic drug historically used for inducing and sustaining basic anesthesia. Current research have drawn consideration to the nonanesthetic results of propofol, however the potential mechanism by which propofol suppresses non-small-cell lung most cancers (NSCLC) development has not been absolutely elucidated.

Strategies: For the in vitro experiments, we used propofol (0, 2, 5, and 10 µg/mL) to deal with A549 cells for 1, 4, and 12 hours and Cell Counting Package-8 (CCK-8) to detect proliferation. Apoptosis was measured with circulation cytometry. We additionally transfected A549 cells with an microribonucleic acid-21 (miR-21) mimic or damaging management ribonucleic acid (RNA) duplex and phosphatase and tensin homolog, deleted on chromosome 10 (PTEN)

small interfering ribonucleic acid (siRNA) or damaging management. PTEN, phosphorylated protein kinase B (pAKT), and protein kinase B (AKT) expression have been detected utilizing Western blotting, whereas miR-21 expression was examined by real-time polymerase chain response (RT-PCR). In vivo, nude mice got injections of A549 cells to develop xenograft tumors; Eight days later, the mice have been intraperitoneally injected with propofol (35 mg/kg) or soybean oil. Tumors have been then collected from mice and analyzed by immunohistochemistry and Western blotting.

Outcomes: Propofol inhibited development (1 hour, P = .001; Four hours, P ≤ .0001; 12 hours, P = .0004) and miR-21 expression (P ≤ .0001) and induced apoptosis (1 hour, P = .0022; Four hours, P = .0005; 12 hours, P ≤ .0001) in A549 cells in a time and concentration-dependent method. MiR-21 mimic and PTEN siRNA transfection antagonized the suppressive results of propofol on A549 cells by lowering PTEN protein

expression (imply variations [MD] [95% confidence interval {CI}], -0.51 [-0.86 to 0.16], P = .0058; MD [95% CI], 0.81 [0.07-1.55], P = .0349, respectively), leading to a rise in pAKT ranges (MD [95% CI] = -0.82 [-1.46 to -0.18], P = .0133) following propofol publicity. In vivo, propofol therapy diminished NSCLC tumor development (MD [95% CI] = -109.47 [-167.03 to -51.91], P ≤ .0001) and promoted apoptosis (MD [95% CI] = 38.53 [11.69-65.36], P = .0093).

Conclusions: Our research indicated that propofol inhibited A549 cell development, accelerated apoptosis by way of the miR-21/PTEN/AKT pathway in vitro, suppressed NSCLC tumor cell development, and promoted apoptosis in vivo. Our findings present new implications for propofol in most cancers remedy and point out that propofol is extraordinarily advantageous in surgical therapy.

Conditional reprogramming: subsequent expertise cell tradition

Prolonged-term main custom of mammalian cells has been always robust attributable to unavoidable senescence. Commonplace methods for producing immortalized cell strains usually require manipulation of genome which leads to change of important natural and genetic traits. Not too way back, conditional reprogramming (CR) emerges as a novel subsequent expertise instrument for long-term custom of main epithelium cells derived from practically all origins with out alteration of genetic background of main cells.

CR co-cultures main cells with inactivated mouse 3T3-J2 fibroblasts inside the presence of RHO-related protein kinase (ROCK) inhibitor Y-27632, enabling main cells to build up stem-like traits whereas retain their potential to fully differentiate. With just some years’ development, CR displays broad prospects in functions in diversified areas along with sickness modeling, regenerative medicine, drug evaluation, drug discovery along with precision medicine. This consider is thus to comprehensively summarize and assess current progress in understanding mechanism of CR and its broad functions, highlighting the value of CR in every main and translational researches and discussing the challenges confronted with CR.

Outcomes of Kifunensine on Manufacturing and N-Glycosylation Modification of Butyrylcholinesterase in a Transgenic Rice CellCulture Bioreactor

The manufacturing and N-glycosylation of recombinant human butyrylcholinesterase (BChE), a model extraordinarily glycosylated therapeutic protein, in a transgenic rice cell suspension custom dealt with with kifunensine, a strong α-mannosidase I inhibitor, was studied in a 5 L bioreactor.

A media commerce was carried out at day 7 of cultivation by eradicating spent sugar-rich medium (NB+S) and together with current sugar-free (NB-S) medium to induce the rice α-amylase 3D (RAmy3D) promoter to supply rice recombinant human BChE (rrBChE). Using a 1.25X-concentrated sugar-free medium together with an 80% lowered working amount by means of the media commerce led to an entire energetic rrBChE manufacturing diploma of 79 ± 2 µg (g FW)-1 or 7.5 ± 0.4 mg L-1 inside the presence of kifunensine, which was 1.5-times larger than our earlier bioreactor runs using common sugar-free (NB-S) media with no kifunensine treatment.

Importantly, the amount of secreted energetic rrBChE in custom medium was enhanced inside the presence of kifunensine, comprising 44% of your complete energetic rrBChE at day 5 following induction. Coomassie-stained SDS-PAGE gel and Western blot analyses revealed fully completely different electrophoretic migration of purified rrBChE bands with and with out kifunensine treatment, which was attributed to fully completely different N-glycoforms. N-Glycosylation analysis confirmed significantly elevated oligomannose glycans (Man5/6/7/8) in rrBChE dealt with with kifunensine as compared with controls. Nonetheless, the mass-transfer limitation of kifunensine was seemingly the principle function for incomplete inhibition of α-mannosidase I on this bioreactor study.

stemcelldocs
stemcelldocs

EF1a control lentivirus (Hygro)

EF1a-Null-Hygro 1 x107 IFU/ml x 200ul
EUR 418.8
Description: Negative control lentivirus contains a null spacer insert under EF1a promoter, serves as the negative control of lentivurs treatment for the specificity of any target expression effects. It has the hygromycin selection under RSV promoter.

RFP (mRuby3) Control Lentivirus

LV101 4 x 500 ul
EUR 195

CAR negative control: CD28-CD3ζ (GFP-Puro) Lentivirus

CAR-ctr8 1x10^8 IFU/ml x 200ul
EUR 276.5
Description: CAR (CD28) control lentivirus without targeting domain, containing GFP-Puromycin dual selection

RFP (TagRFP-T) Control Lentivirus

LV097 4 x 500 ul
EUR 195

CAR negative control: 4-1BB-CD3ζ (GFP-Puro) Lentivirus

CAR-ctr7 1x10^8 IFU/ml x 200ul
EUR 276.5
Description: CAR (4-1BB) control lentivirus without targeting domain, containing GFP-Puromycin dual selection

Negative Control Luciferase Lentivirus

79578 500 µl x 2
EUR 860
Description: The Negative Control Lentivirus (Firefly Luciferase) are replication incompetent, HIV-based, VSV-G pseudo typed lentiviral particles that are ready to be transduced into almost all types of mammalian cells, including primary and non-dividing cells. The particles contain a firefly luciferase gene under the control of a minimal TATA promoter, without any additional transcriptional response elements.

CMV control lentivirus (No Selection)

CMV-Null- 1 x10^7 IFU/ml x 200ul
EUR 206.5
Description: Negative control lentivirus under CMV promoter, does not contain any antibiotic selection.

Negative Control eGFP Reporter Lentivirus

79927 500 µl x 2
EUR 835
Description: The Negative Control eGFP Reporter Lentivirus are replication incompetent, HIV-based, VSV-G pseudotyped lentiviral particles that are ready to infect almost all types of mammalian cells, including primary and non-dividing cells. The particles contain an enhanced Green Fluorescent Protein (eGFP) gene under the control of a minimal TATA promoter, without any additional transcriptional response elements.

Expression Negative Control Lentivirus (G418)

79902-G 500 µl x 2
EUR 795
Description: The Expression Negative Control Lentivirus are replication incompetent, HIV-based, VSV-G pseudotyped lentiviral particles that are ready to be transduced into almost all types of mammalian cells, including primary and non-dividing cells. The controls package the same virus particles as the target expression virus, but they do not express a specific protein under the CMV promoter. _x000D_The Expression Negative Control Lentivirus (G418) expresses the gene for aminoglycoside 3' phosphotransferase, which confers resistance to kanamycin, neomycin, and geneticin (G418)._x000D_The Expression Negative Control Lentivirus (Hygromycin) expresses the gene for hygromycin B phosphotransferase, which confers resistance to Hygromycin._x000D_The Expression Negative Control Lentivirus (Puromycin) expresses the gene for puromycin N-acetyl-transferase, which confers resistance to puromycin._x000D_ _x000D_

Expression Negative Control Lentivirus (Puromycin)

79902-P 500 µl x 2
EUR 795
Description: The Expression Negative Control Lentivirus are replication incompetent, HIV-based, VSV-G pseudotyped lentiviral particles that are ready to be transduced into almost all types of mammalian cells, including primary and non-dividing cells. The controls package the same virus particles as the target expression virus, but they do not express a specific protein under the CMV promoter. _x000D_The Expression Negative Control Lentivirus (G418) expresses the gene for aminoglycoside 3' phosphotransferase, which confers resistance to kanamycin, neomycin, and geneticin (G418)._x000D_The Expression Negative Control Lentivirus (Hygromycin) expresses the gene for hygromycin B phosphotransferase, which confers resistance to Hygromycin._x000D_The Expression Negative Control Lentivirus (Puromycin) expresses the gene for puromycin N-acetyl-transferase, which confers resistance to puromycin._x000D_ _x000D_

Expression Negative Control Lentivirus (Hygromycin)

79902-H 500 µl x 2
EUR 795
Description: The Expression Negative Control Lentivirus are replication incompetent, HIV-based, VSV-G pseudotyped lentiviral particles that are ready to be transduced into almost all types of mammalian cells, including primary and non-dividing cells. The controls package the same virus particles as the target expression virus, but they do not express a specific protein under the CMV promoter. _x000D_The Expression Negative Control Lentivirus (G418) expresses the gene for aminoglycoside 3' phosphotransferase, which confers resistance to kanamycin, neomycin, and geneticin (G418)._x000D_The Expression Negative Control Lentivirus (Hygromycin) expresses the gene for hygromycin B phosphotransferase, which confers resistance to Hygromycin._x000D_The Expression Negative Control Lentivirus (Puromycin) expresses the gene for puromycin N-acetyl-transferase, which confers resistance to puromycin._x000D_ _x000D_

CAR negative control: CD28-CD3ζ (No Select) Lentivirus

CAR-ctr6 1x10^8 IFU/ml x 200ul
EUR 276.5
Description: CAR (CD28) control lentivirus without targeting domain, does not contain any antibiotic selection

CMV Control (No Antibiotic), Concentrated Lentivirus in PBS

CMV-Null-PBS 1 x10^8 IFU/ml x 200ul
EUR 455
Description: Concentrated Negative control lentivirus under CMV promoter, contains no any antibioic selection.

EF1a Control (No Antibiotic), Concentrated Lentivirus in PBS

EF1a-Null-PBS 1 x10^8 IFU/ml x 200ul
EUR 455
Description: Concentrated Negative control lentivirus under EF1a promoter, contains no any antibioic selection.

CAR negative control: 4-1BB-CD3ζ (No Select) Lentivirus

CAR-ctr5 1x10^8 IFU/ml x 200ul
EUR 276.5
Description: CAR (4-1BB) control lentivirus without targeting domain, do not any antibiotic selection

AR-RFP (GFP) Lentivirus 

LVP913-G 1x107 IFU/ml x 200ul
EUR 662.4
Description: Pre-made lentivirus express RFP reporter under the minimal promoter contains 4 tandem repeats of Androgen Response Element (ARE). This lentivirus also contain the GFP selection marker under the consitutive RSV promoter, provided in DMEM medium with 10% FBS and 60ug/ml of polybrene.

AR-Luc (GFP) Lentivirus 

LVP914-G 1x107 IFU/ml x 200ul
EUR 662.4
Description: Pre-made lentivirus express Firefly luciferase reporter under the minimal promoter contains 4 tandem repeats of Androgen Response Element (ARE). This lentivirus also contain the GFP selection marker under the consitutiveRSV promoter, provided in DMEM medium with 10% FBS and 60ug/ml of polybrene.

G_RE-RFP (GFP) Lentivirus 

LVP950-G 1x107 IFU/ml x 200ul
EUR 662.4
Description: Pre-made lentivirus express RFP reporter under a minimal promoter embedded 4 tandem repeats of glucocorticoid response element (G-RE). This lentivirus also contain the GFP selection marker under the consitutive RSV promoter, provided in DMEM medium with 10% FBS and 60ug/ml of polybrene.

G_RE-Luc (GFP) Lentivirus 

LVP951-G 1x107 IFU/ml x 200ul
EUR 662.4
Description: Pre-made lentivirus express Firefly luciferase reporter under a minimal promoter embedded 4 tandem repeats of glucocorticoid response element (G-RE). This lentivirus also contain the GFP selection marker under the consitutiveRSV promoter, provided in DMEM medium with 10% FBS and 60ug/ml of polybrene.

H_RE-RFP (GFP) Lentivirus 

LVP970-G 1x107 IFU/ml x 200ul
EUR 662.4
Description: Pre-made lentivirus express RFP reporter under a minimal promoter that embedded 6 tandem repeats of hypoxia transcriptional response element (H-RE). This lentivirus also contain the GFP selection marker under the consitutive RSV promoter, provided in DMEM medium with 10% FBS and 60ug/ml of polybrene.

H_RE-Luc (GFP) Lentivirus 

LVP971-G 1x107 IFU/ml x 200ul
EUR 662.4
Description: Pre-made lentivirus express Firefly luciferase reporter under a minimal promoter that embedded 6 tandem repeats of hypoxia transcriptional response element (H-RE). This lentivirus also contain the GFP selection marker under the consitutiveRSV promoter, provided in DMEM medium with 10% FBS and 60ug/ml of polybrene.

ER-RFP (GFP) Lentivirus 

LVP974-G 1x107 IFU/ml x 200ul
EUR 662.4
Description: Pre-made lentivirus express RFP reporter under a minimal promoter that embedded 4 tandem repeats of estrogen receptor response element (ER-RE). This lentivirus also contain the GFP selection marker under the consitutive RSV promoter, provided in DMEM medium with 10% FBS and 60ug/ml of polybrene.

ER-Luc (GFP) Lentivirus 

LVP975-G 1x107 IFU/ml x 200ul
EUR 662.4
Description: Pre-made lentivirus express Firefly luciferase reporter under a minimal promoter that embedded 4 tandem repeats of estrogen receptor response element (ER-RE). This lentivirus also contain the GFP selection marker under the consitutiveRSV promoter, provided in DMEM medium with 10% FBS and 60ug/ml of polybrene.

MB-RFP (GFP) Lentivirus 

LVP1022-G 1x107 IFU/ml x 200ul
EUR 662.4
Description: Pre-made lentivirus express RFP reporter under human Myoglobin 's promoter which only expressed in muscle, predominantly in cardiac and skeletal myocytes. This lentivirus also contain the GFP selection marker under the consitutive RSV promoter, provided in DMEM medium with 10% FBS and 60ug/ml of polybrene.

MB-Luc (GFP) Lentivirus 

LVP1023-G 1x107 IFU/ml x 200ul
EUR 662.4
Description: Pre-made lentivirus express Firefly luciferase reporter under human Myoglobin 's promoter which only expressed in muscle, predominantly in cardiac and skeletal myocytes. This lentivirus also contain the GFP selection marker under the consitutiveRSV promoter, provided in DMEM medium with 10% FBS and 60ug/ml of polybrene.

HE4-RFP (GFP) Lentivirus 

LVP1042-G 1x107 IFU/ml x 200ul
EUR 662.4
Description: Pre-made lentivirus express RFP reporter under human HE4's promoter which is overexpressed in ovarian cancer cells. This lentivirus also contain the GFP selection marker under the consitutive RSV promoter, provided in DMEM medium with 10% FBS and 60ug/ml of polybrene.

HE4-Luc (GFP) Lentivirus 

LVP1043-G 1x107 IFU/ml x 200ul
EUR 662.4
Description: Pre-made lentivirus express Firefly luciferase reporter under human HE4's promoter which is overexpressed in ovarian cancer cells. This lentivirus also contain the GFP selection marker under the consitutiveRSV promoter, provided in DMEM medium with 10% FBS and 60ug/ml of polybrene.

PSA-RFP (GFP) Lentivirus 

LVP1046-G 1x107 IFU/ml x 200ul
EUR 662.4
Description: Pre-made lentivirus express RFP reporter under human PSA's promoter which expressed in normal prostate epithelium and prostate cancers. This lentivirus also contain the GFP selection marker under the consitutive RSV promoter, provided in DMEM medium with 10% FBS and 60ug/ml of polybrene.

PSA-Luc (GFP) Lentivirus 

LVP1047-G 1x107 IFU/ml x 200ul
EUR 662.4
Description: Pre-made lentivirus express Firefly luciferase reporter under human PSA's promoter which expressed in normal prostate epithelium and prostate cancers. This lentivirus also contain the GFP selection marker under the consitutiveRSV promoter, provided in DMEM medium with 10% FBS and 60ug/ml of polybrene.

AR-Rluc (GFP) Lentivirus 

LVP915-G 1x107 IFU/ml x 200ul
EUR 662.4
Description: Pre-made lentivirus express Renilla luciferase reporter under the minimal promoter contains 4 tandem repeats of Androgen Response Element (ARE). This lentivirus also contain the GFP selection marker under the consitutive RSV promoter, provided in DMEM medium with 10% FBS and 60ug/ml of polybrene.

Gli-RFP (GFP) Lentivirus 

LVP946-G 1x107 IFU/ml x 200ul
EUR 662.4
Description: Pre-made lentivirus express RFP reporter under a minimal promoter embeded 8 tandem repeats of Gli responsive element (hedgehog pathway). This lentivirus also contain the GFP selection marker under the consitutive RSV promoter, provided in DMEM medium with 10% FBS and 60ug/ml of polybrene.

Gli-Luc (GFP) Lentivirus 

LVP947-G 1x107 IFU/ml x 200ul
EUR 662.4
Description: Pre-made lentivirus express Firefly luciferase reporter under a minimal promoter embeded 8 tandem repeats of Gli responsive element (hedgehog pathway). This lentivirus also contain the GFP selection marker under the consitutiveRSV promoter, provided in DMEM medium with 10% FBS and 60ug/ml of polybrene.

G_RE-Rluc (GFP) Lentivirus 

LVP952-G 1x107 IFU/ml x 200ul
EUR 662.4
Description: Pre-made lentivirus express Renilla luciferase reporter under a minimal promoter embedded 4 tandem repeats of glucocorticoid response element (G-RE). This lentivirus also contain the GFP selection marker under the consitutive RSV promoter, provided in DMEM medium with 10% FBS and 60ug/ml of polybrene.

AP1-RFP (GFP) Lentivirus 

LVP954-G 1x107 IFU/ml x 200ul
EUR 662.4
Description: Pre-made lentivirus express RFP reporter under a minimal promoter that embedded 8 tandem repeats of AP1 Responsive Element (AP1-RE) . This lentivirus also contain the GFP selection marker under the consitutive RSV promoter, provided in DMEM medium with 10% FBS and 60ug/ml of polybrene.

AP1-Luc (GFP) Lentivirus 

LVP955-G 1x107 IFU/ml x 200ul
EUR 662.4
Description: Pre-made lentivirus express Firefly luciferase reporter under a minimal promoter that embedded 8 tandem repeats of AP1 Responsive Element (AP1-RE) . This lentivirus also contain the GFP selection marker under the consitutiveRSV promoter, provided in DMEM medium with 10% FBS and 60ug/ml of polybrene.

SRE-RFP (GFP) Lentivirus 

LVP958-G 1x107 IFU/ml x 200ul
EUR 662.4
Description: Pre-made lentivirus express RFP reporter under a minimal promoter that embedded 4 tandem repeats of serum response element (SRE) . This lentivirus also contain the GFP selection marker under the consitutive RSV promoter, provided in DMEM medium with 10% FBS and 60ug/ml of polybrene.

SRE-Luc (GFP) Lentivirus 

LVP959-G 1x107 IFU/ml x 200ul
EUR 662.4
Description: Pre-made lentivirus express Firefly luciferase reporter under a minimal promoter that embedded 4 tandem repeats of serum response element (SRE) . This lentivirus also contain the GFP selection marker under the consitutiveRSV promoter, provided in DMEM medium with 10% FBS and 60ug/ml of polybrene.

RBP_JK-RFP (GFP) Lentivirus 

LVP962-G 1x107 IFU/ml x 200ul
EUR 662.4
Description: Pre-made lentivirus express RFP reporter under a minimal promoter that embedded 4 tandem repeats of RBP-JK transcriptional responsive element (RBP-JK TRE). This lentivirus also contain the GFP selection marker under the consitutive RSV promoter, provided in DMEM medium with 10% FBS and 60ug/ml of polybrene.

RBP_JK-Luc (GFP) Lentivirus 

LVP963-G 1x107 IFU/ml x 200ul
EUR 662.4
Description: Pre-made lentivirus express Firefly luciferase reporter under a minimal promoter that embedded 4 tandem repeats of RBP-JK transcriptional responsive element (RBP-JK TRE). This lentivirus also contain the GFP selection marker under the consitutiveRSV promoter, provided in DMEM medium with 10% FBS and 60ug/ml of polybrene.

H_RE-Rluc (GFP) Lentivirus 

LVP972-G 1x107 IFU/ml x 200ul
EUR 662.4
Description: Pre-made lentivirus express Renilla luciferase reporter under a minimal promoter that embedded 6 tandem repeats of hypoxia transcriptional response element (H-RE). This lentivirus also contain the GFP selection marker under the consitutive RSV promoter, provided in DMEM medium with 10% FBS and 60ug/ml of polybrene.

ER-Rluc (GFP) Lentivirus 

LVP976-G 1x107 IFU/ml x 200ul
EUR 662.4
Description: Pre-made lentivirus express Renilla luciferase reporter under a minimal promoter that embedded 4 tandem repeats of estrogen receptor response element (ER-RE). This lentivirus also contain the GFP selection marker under the consitutive RSV promoter, provided in DMEM medium with 10% FBS and 60ug/ml of polybrene.

P53-RFP (GFP) Lentivirus 

LVP978-G 1x107 IFU/ml x 200ul
EUR 662.4
Description: Pre-made lentivirus express RFP reporter under a minimal promoter that embedded with optimized tandem repeats of a few most potent P53 transcriptional response element. This lentivirus also contain the GFP selection marker under the consitutive RSV promoter, provided in DMEM medium with 10% FBS and 60ug/ml of polybrene.

P53-Luc (GFP) Lentivirus 

LVP979-G 1x107 IFU/ml x 200ul
EUR 662.4
Description: Pre-made lentivirus express Firefly luciferase reporter under a minimal promoter that embedded with optimized tandem repeats of a few most potent P53 transcriptional response element. This lentivirus also contain the GFP selection marker under the consitutiveRSV promoter, provided in DMEM medium with 10% FBS and 60ug/ml of polybrene.

ARE-RFP (GFP) Lentivirus 

LVP982-G 1x107 IFU/ml x 200ul
EUR 662.4
Description: Pre-made lentivirus express RFP reporter under a minimal promoter that embedded 4 tandem repeats of antioxidant response element (ARE). This lentivirus also contain the GFP selection marker under the consitutive RSV promoter, provided in DMEM medium with 10% FBS and 60ug/ml of polybrene.

ARE-Luc (GFP) Lentivirus 

LVP983-G 1x107 IFU/ml x 200ul
EUR 662.4
Description: Pre-made lentivirus express Firefly luciferase reporter under a minimal promoter that embedded 4 tandem repeats of antioxidant response element (ARE). This lentivirus also contain the GFP selection marker under the consitutiveRSV promoter, provided in DMEM medium with 10% FBS and 60ug/ml of polybrene.

B29-RFP (GFP) Lentivirus 

LVP994-G 1x107 IFU/ml x 200ul
EUR 662.4
Description: Pre-made lentivirus express RFP reporter under a human Immunoglobulin gene's promoter which demonstrates B-cell specific expression. This lentivirus also contain the GFP selection marker under the consitutive RSV promoter, provided in DMEM medium with 10% FBS and 60ug/ml of polybrene.

B29-Luc (GFP) Lentivirus 

LVP995-G 1x107 IFU/ml x 200ul
EUR 662.4
Description: Pre-made lentivirus express Firefly luciferase reporter under a human Immunoglobulin gene's promoter which demonstrates B-cell specific expression. This lentivirus also contain the GFP selection marker under the consitutiveRSV promoter, provided in DMEM medium with 10% FBS and 60ug/ml of polybrene.

MB-Rluc (GFP) Lentivirus 

LVP1024-G 1x107 IFU/ml x 200ul
EUR 662.4
Description: Pre-made lentivirus express Renilla luciferase reporter under human Myoglobin 's promoter which only expressed in muscle, predominantly in cardiac and skeletal myocytes. This lentivirus also contain the GFP selection marker under the consitutive RSV promoter, provided in DMEM medium with 10% FBS and 60ug/ml of polybrene.

SPB-RFP (GFP) Lentivirus 

LVP1026-G 1x107 IFU/ml x 200ul
EUR 662.4
Description: Pre-made lentivirus express RFP reporter under human Surfactant protein B's promoter which selectively expressed in bronchiolar and alveolar epithelial cells of the lung. This lentivirus also contain the GFP selection marker under the consitutive RSV promoter, provided in DMEM medium with 10% FBS and 60ug/ml of polybrene.

SPB-Luc (GFP) Lentivirus 

LVP1027-G 1x107 IFU/ml x 200ul
EUR 662.4
Description: Pre-made lentivirus express Firefly luciferase reporter under human Surfactant protein B's promoter which selectively expressed in bronchiolar and alveolar epithelial cells of the lung. This lentivirus also contain the GFP selection marker under the consitutiveRSV promoter, provided in DMEM medium with 10% FBS and 60ug/ml of polybrene.

aFP-RFP (GFP) Lentivirus 

LVP1034-G 1x107 IFU/ml x 200ul
EUR 662.4
Description: Pre-made lentivirus express RFP reporter under human alpha-fetoprotein 's promoter which used for the expression into hepatocellular carcinoma cells . This lentivirus also contain the GFP selection marker under the consitutive RSV promoter, provided in DMEM medium with 10% FBS and 60ug/ml of polybrene.

aFP-Luc (GFP) Lentivirus 

LVP1035-G 1x107 IFU/ml x 200ul
EUR 662.4
Description: Pre-made lentivirus express Firefly luciferase reporter under human alpha-fetoprotein 's promoter which used for the expression into hepatocellular carcinoma cells . This lentivirus also contain the GFP selection marker under the consitutiveRSV promoter, provided in DMEM medium with 10% FBS and 60ug/ml of polybrene.

aFP-Rluc (GFP) Lentivirus 

LVP1036-G 1x107 IFU/ml x 200ul
EUR 662.4
Description: Pre-made lentivirus express Renilla luciferase reporter under human alpha-fetoprotein 's promoter which used for the expression into hepatocellular carcinoma cells . This lentivirus also contain the GFP selection marker under the consitutive RSV promoter, provided in DMEM medium with 10% FBS and 60ug/ml of polybrene.

HE4-Rluc (GFP) Lentivirus 

LVP1044-G 1x107 IFU/ml x 200ul
EUR 662.4
Description: Pre-made lentivirus express Renilla luciferase reporter under human HE4's promoter which is overexpressed in ovarian cancer cells. This lentivirus also contain the GFP selection marker under the consitutive RSV promoter, provided in DMEM medium with 10% FBS and 60ug/ml of polybrene.

PSA-Rluc (GFP) Lentivirus 

LVP1048-G 1x107 IFU/ml x 200ul
EUR 662.4
Description: Pre-made lentivirus express Renilla luciferase reporter under human PSA's promoter which expressed in normal prostate epithelium and prostate cancers. This lentivirus also contain the GFP selection marker under the consitutive RSV promoter, provided in DMEM medium with 10% FBS and 60ug/ml of polybrene.

EGR1-RFP (GFP) Lentivirus 

LVP1120-G 1x107 IFU/ml x 200ul
EUR 662.4
Description: Pre-made lentivirus express RFP reporter under the promoter of human early growth response gene-1 (EGR1). This lentivirus contains another fluorescent marker GFP under RSV promoter, provided in DMEM medium with 10% FBS and 60ug/ml of polybrene.

EGR1-RFP (GFP) Lentivirus 

LVP1120-G-PBS 1x108 IFU/ml x 200ul
EUR 852
Description: Pre-made lentivirus express RFP reporter under the promoter of human early growth response gene-1 (EGR1). This lentivirus contains another fluorescent marker GFP under RSV promoter, provided in PBS solution.

EGR1-Luc (GFP) Lentivirus 

LVP1121-G 1x107 IFU/ml x 200ul
EUR 662.4
Description: Pre-made lentivirus express Firefly Luciferase reporter under the promoter of human early growth response gene-1 (EGR1). This lentivirus contains the fluorescent marker GFP under RSV promoter, provided in DMEM medium with 10% FBS and 60ug/ml of polybrene.

EGR1-Luc (GFP) Lentivirus 

LVP1121-G-PBS 1x108 IFU/ml x 200ul
EUR 852
Description: Pre-made lentivirus express Firefly Luciferase reporter under the promoter of human early growth response gene-1 (EGR1). This lentivirus contains the fluorescent marker GFP under RSV promoter, provided in PBS solution.

GFAP-RFP (GFP) Lentivirus 

LVP1126-G 1x107 IFU/ml x 200ul
EUR 662.4
Description: Pre-made lentivirus express RFP reporter under human GFAP's promoter which expressed in astrocytes in Central Nervous System. This lentivirus also contain the GFP selection marker under the consitutive RSV promoter, provided in DMEM medium with 10% FBS and 60ug/ml of polybrene.

GFAP-RFP (GFP) Lentivirus 

LVP1126-G-PBS 1x108 IFU/ml x 200ul
EUR 852
Description: Pre-made lentivirus express RFP reporter under human GFAP's promoter which expressed in astrocytes in Central Nervous System. This lentivirus also contain the GFP selection marker under the consitutive RSV promoter. Lentivirus concentrated and provided in PBS solution.

GFAP-Luc (GFP) Lentivirus 

LVP1127-G 1x107 IFU/ml x 200ul
EUR 662.4
Description: Pre-made lentivirus express Firefly luciferase reporter under human GFAP's promoter which expressed in astrocytes in Central Nervous System. This lentivirus also contain the GFP selection marker under the consitutiveRSV promoter, provided in DMEM medium with 10% FBS and 60ug/ml of polybrene.

GFAP-Luc (GFP) Lentivirus 

LVP1127-G-PBS 1x108 IFU/ml x 200ul
EUR 852
Description: Pre-made lentivirus express Firefly luciferase reporter under human GFAP's promoter which expressed in astrocytes in Central Nervous System. This lentivirus also contain the GFP selection marker under the consitutiveRSV promoter. Lentivirus concentrated and provided in PBS solution.

ISRE-RFP (GFP) Lentivirus 

LVP938-G 1x107 IFU/ml x 200ul
EUR 662.4
Description: Pre-made lentivirus express RFP reporter undera minimal promoter embeded 8 tandem repeats of ISRE (Interferon Stimulated Response Element). This lentivirus also contain the GFP selection marker under the consitutive RSV promoter, provided in DMEM medium with 10% FBS and 60ug/ml of polybrene.

ISRE-Luc (GFP) Lentivirus 

LVP939-G 1x107 IFU/ml x 200ul
EUR 662.4
Description: Pre-made lentivirus express Firefly luciferase reporter undera minimal promoter embeded 8 tandem repeats of ISRE (Interferon Stimulated Response Element). This lentivirus also contain the GFP selection marker under the consitutiveRSV promoter, provided in DMEM medium with 10% FBS and 60ug/ml of polybrene.

CRE_TRE-RFP (GFP) Lentivirus 

LVP942-G 1x107 IFU/ml x 200ul
EUR 662.4
Description: Pre-made lentivirus express RFP reporter under a minimal promoter embeded 8 tandem repeats of cAMP response motif (CRE). This lentivirus also contain the GFP selection marker under the consitutive RSV promoter, provided in DMEM medium with 10% FBS and 60ug/ml of polybrene.

CRE_TRE-Luc (GFP) Lentivirus 

LVP943-G 1x107 IFU/ml x 200ul
EUR 662.4
Description: Pre-made lentivirus express Firefly luciferase reporter under a minimal promoter embeded 8 tandem repeats of cAMP response motif (CRE). This lentivirus also contain the GFP selection marker under the consitutiveRSV promoter, provided in DMEM medium with 10% FBS and 60ug/ml of polybrene.

Gli-Rluc (GFP) Lentivirus 

LVP948-G 1x107 IFU/ml x 200ul
EUR 662.4
Description: Pre-made lentivirus express Renilla luciferase reporter under a minimal promoter embeded 8 tandem repeats of Gli responsive element (hedgehog pathway). This lentivirus also contain the GFP selection marker under the consitutive RSV promoter, provided in DMEM medium with 10% FBS and 60ug/ml of polybrene.

AP1-Rluc (GFP) Lentivirus 

LVP956-G 1x107 IFU/ml x 200ul
EUR 662.4
Description: Pre-made lentivirus express Renilla luciferase reporter under a minimal promoter that embedded 8 tandem repeats of AP1 Responsive Element (AP1-RE) . This lentivirus also contain the GFP selection marker under the consitutive RSV promoter, provided in DMEM medium with 10% FBS and 60ug/ml of polybrene.

SRE-Rluc (GFP) Lentivirus 

LVP960-G 1x107 IFU/ml x 200ul
EUR 662.4
Description: Pre-made lentivirus express Renilla luciferase reporter under a minimal promoter that embedded 4 tandem repeats of serum response element (SRE) . This lentivirus also contain the GFP selection marker under the consitutive RSV promoter, provided in DMEM medium with 10% FBS and 60ug/ml of polybrene.

RBP_JK-Rluc (GFP) Lentivirus 

LVP964-G 1x107 IFU/ml x 200ul
EUR 662.4
Description: Pre-made lentivirus express Renilla luciferase reporter under a minimal promoter that embedded 4 tandem repeats of RBP-JK transcriptional responsive element (RBP-JK TRE). This lentivirus also contain the GFP selection marker under the consitutive RSV promoter, provided in DMEM medium with 10% FBS and 60ug/ml of polybrene.

NFkB-RFP (GFP) Lentivirus 

LVP966-G 1x107 IFU/ml x 200ul
EUR 662.4
Description: Pre-made lentivirus express RFP reporter under a minimal promoter that embedded 4 tandem repeats of NFκB transcriptional response element (NFkB-TRE). This lentivirus also contain the GFP selection marker under the consitutive RSV promoter, provided in DMEM medium with 10% FBS and 60ug/ml of polybrene.

NFkB-Luc (GFP) Lentivirus 

LVP967-G 1x107 IFU/ml x 200ul
EUR 662.4
Description: Pre-made lentivirus express Firefly luciferase reporter under a minimal promoter that embedded 4 tandem repeats of NFκB transcriptional response element (NFkB-TRE). This lentivirus also contain the GFP selection marker under the consitutiveRSV promoter, provided in DMEM medium with 10% FBS and 60ug/ml of polybrene.

P53-Rluc (GFP) Lentivirus 

LVP980-G 1x107 IFU/ml x 200ul
EUR 662.4
Description: Pre-made lentivirus express Renilla luciferase reporter under a minimal promoter that embedded with optimized tandem repeats of a few most potent P53 transcriptional response element. This lentivirus also contain the GFP selection marker under the consitutive RSV promoter, provided in DMEM medium with 10% FBS and 60ug/ml of polybrene.

ARE-Rluc (GFP) Lentivirus 

LVP984-G 1x107 IFU/ml x 200ul
EUR 662.4
Description: Pre-made lentivirus express Renilla luciferase reporter under a minimal promoter that embedded 4 tandem repeats of antioxidant response element (ARE). This lentivirus also contain the GFP selection marker under the consitutive RSV promoter, provided in DMEM medium with 10% FBS and 60ug/ml of polybrene.

CEBP-RFP (GFP) Lentivirus 

LVP986-G 1x107 IFU/ml x 200ul
EUR 662.4
Description: Pre-made lentivirus express RFP reporter under a minimal promoter that embedded 8 tandem repeats of C/EBP transcriptional response element (C/EBP-TRE). This lentivirus also contain the GFP selection marker under the consitutive RSV promoter, provided in DMEM medium with 10% FBS and 60ug/ml of polybrene.

CEBP-Luc (GFP) Lentivirus 

LVP987-G 1x107 IFU/ml x 200ul
EUR 662.4
Description: Pre-made lentivirus express Firefly luciferase reporter under a minimal promoter that embedded 8 tandem repeats of C/EBP transcriptional response element (C/EBP-TRE). This lentivirus also contain the GFP selection marker under the consitutiveRSV promoter, provided in DMEM medium with 10% FBS and 60ug/ml of polybrene.

B29-Rluc (GFP) Lentivirus 

LVP996-G 1x107 IFU/ml x 200ul
EUR 662.4
Description: Pre-made lentivirus express Renilla luciferase reporter under a human Immunoglobulin gene's promoter which demonstrates B-cell specific expression. This lentivirus also contain the GFP selection marker under the consitutive RSV promoter, provided in DMEM medium with 10% FBS and 60ug/ml of polybrene.

CD14-RFP (GFP) Lentivirus 

LVP998-G 1x107 IFU/ml x 200ul
EUR 662.4
Description: Pre-made lentivirus express RFP reporter under the human CD14 promoter which demonstrates strongly upregulated expression during monocytic cell differentiation. This lentivirus also contain the GFP selection marker under the consitutive RSV promoter, provided in DMEM medium with 10% FBS and 60ug/ml of polybrene.

CD14-Luc (GFP) Lentivirus 

LVP999-G 1x107 IFU/ml x 200ul
EUR 662.4
Description: Pre-made lentivirus express Firefly luciferase reporter under the human CD14 promoter which demonstrates strongly upregulated expression during monocytic cell differentiation. This lentivirus also contain the GFP selection marker under the consitutiveRSV promoter, provided in DMEM medium with 10% FBS and 60ug/ml of polybrene.

CD43-RFP (GFP) Lentivirus 

LVP1002-G 1x107 IFU/ml x 200ul
EUR 662.4
Description: Pre-made lentivirus express RFP reporter under the human CD43 promoter which expressed on the surface of leukocytes and platelets.. This lentivirus also contain the GFP selection marker under the consitutive RSV promoter, provided in DMEM medium with 10% FBS and 60ug/ml of polybrene.

CD43-Luc (GFP) Lentivirus 

LVP1003-G 1x107 IFU/ml x 200ul
EUR 662.4
Description: Pre-made lentivirus express Firefly luciferase reporter under the human CD43 promoter which expressed on the surface of leukocytes and platelets.. This lentivirus also contain the GFP selection marker under the consitutiveRSV promoter, provided in DMEM medium with 10% FBS and 60ug/ml of polybrene.

CD45-RFP (GFP) Lentivirus 

LVP1006-G 1x107 IFU/ml x 200ul
EUR 662.4
Description: Pre-made lentivirus express RFP reporter under the human CD45's promoter which expressed exclusively by all hematopoietic cells except erythrocytes and platelets. This lentivirus also contain the GFP selection marker under the consitutive RSV promoter, provided in DMEM medium with 10% FBS and 60ug/ml of polybrene.

CD45-Luc (GFP) Lentivirus 

LVP1007-G 1x107 IFU/ml x 200ul
EUR 662.4
Description: Pre-made lentivirus express Firefly luciferase reporter under the human CD45's promoter which expressed exclusively by all hematopoietic cells except erythrocytes and platelets. This lentivirus also contain the GFP selection marker under the consitutiveRSV promoter, provided in DMEM medium with 10% FBS and 60ug/ml of polybrene.

CD68-RFP (GFP) Lentivirus 

LVP1010-G 1x107 IFU/ml x 200ul
EUR 662.4
Description: Pre-made lentivirus express RFP reporter under the human CD68's promoter which expressed specifically in macrophages and macrophage-related cells. This lentivirus also contain the GFP selection marker under the consitutive RSV promoter, provided in DMEM medium with 10% FBS and 60ug/ml of polybrene.

CD68-Luc (GFP) Lentivirus 

LVP1011-G 1x107 IFU/ml x 200ul
EUR 662.4
Description: Pre-made lentivirus express Firefly luciferase reporter under the human CD68's promoter which expressed specifically in macrophages and macrophage-related cells. This lentivirus also contain the GFP selection marker under the consitutiveRSV promoter, provided in DMEM medium with 10% FBS and 60ug/ml of polybrene.

Flt1-RFP (GFP) Lentivirus 

LVP1014-G 1x107 IFU/ml x 200ul
EUR 662.4
Description: Pre-made lentivirus express RFP reporter under the human Flt-1's promoter which expressed specifically in endothelial cells and up-regulated in tumor vasculature. This lentivirus also contain the GFP selection marker under the consitutive RSV promoter, provided in DMEM medium with 10% FBS and 60ug/ml of polybrene.

Flt1-Luc (GFP) Lentivirus 

LVP1015-G 1x107 IFU/ml x 200ul
EUR 662.4
Description: Pre-made lentivirus express Firefly luciferase reporter under the human Flt-1's promoter which expressed specifically in endothelial cells and up-regulated in tumor vasculature. This lentivirus also contain the GFP selection marker under the consitutiveRSV promoter, provided in DMEM medium with 10% FBS and 60ug/ml of polybrene.

SPB-Rluc (GFP) Lentivirus 

LVP1028-G 1x107 IFU/ml x 200ul
EUR 662.4
Description: Pre-made lentivirus express Renilla luciferase reporter under human Surfactant protein B's promoter which selectively expressed in bronchiolar and alveolar epithelial cells of the lung. This lentivirus also contain the GFP selection marker under the consitutive RSV promoter, provided in DMEM medium with 10% FBS and 60ug/ml of polybrene.

SYN1-RFP (GFP) Lentivirus 

LVP1030-G 1x107 IFU/ml x 200ul
EUR 662.4
Description: Pre-made lentivirus express RFP reporter under human Synapsin 's promoter which used for neuron-specific high expression. This lentivirus also contain the GFP selection marker under the consitutive RSV promoter, provided in DMEM medium with 10% FBS and 60ug/ml of polybrene.

Self-Organizing Human Induced Pluripotent Stem Cell Hepatocyte 3D Organoids Inform the Biology of the Pleiotropic TRIB1 Gene

Institution of a physiologically related human hepatocyte-like cell system for in vitro translational analysis has been hampered by the restricted availability of cell fashions that precisely replicate human biology and the pathophysiology of human illness. Right here we report a strong, reproducible, and scalable protocol for the technology of hepatic organoids from human induced pluripotent stem cells (hiPSCs) utilizing brief publicity to nonengineered matrices.

These hepatic organoids observe outlined levels of hepatic improvement and categorical greater ranges of early (hepatocyte nuclear issue 4A [HNF4A], prospero-related homeobox 1 [PROX1]) and mature hepatic and metabolic markers (albumin, asialoglycoprotein receptor 1 [ASGR1], CCAAT/enhancer binding protein α [C/EBPα]) than two-dimensional (2D) hepatocyte-like cells (HLCs) at day 20 of differentiation.

We used this mannequin to discover the biology of the pleiotropic TRIB1 (Tribbles-1) gene related to a variety of metabolic traits, together with nonalcoholic fatty liver illness and plasma lipids.

We used genome enhancing to delete the TRIB1 gene in hiPSCs and in contrast TRIB1-deleted iPSC-HLCs to isogenic iPSC-HLCs underneath each 2D tradition and three-dimensional (3D) organoid circumstances. Beneath standard 2D tradition circumstances, TRIB1-deficient HLCs confirmed maturation defects, with decreased expression of late-stage hepatic and lipogenesis markers.

In distinction, when cultured as 3D hepatic organoids, the differentiation defects have been rescued, and a transparent lipid-related phenotype was famous within the TRIB1-deficient induced pluripotent stem cell HLCs. Conclusion: This work helps the potential of genome-edited hiPSC-derived hepatic 3D organoids in exploring human hepatocyte biology, together with the practical interrogation of genes recognized by means of human genetic investigation.

293/Cas9 Cell Line

AKR-5110 1 vial
EUR 460

HeLa/Cas9 Cell Line

AKR-5111 1 vial
EUR 460

NIH3T3/Cas9 Cell Line

AKR-5104 1 vial
EUR 460

MCF7 [MCF-7] Cell Line

CL-0149 1×10⁶ cells/vial
EUR 420
Description: Homo sapiens, Human

SKOV-3/Luc Cell Line

AKR-232 1 vial
EUR 686.4
Description: SKOV-3/Luc Cell Line stably expresses luciferase and otherwise exhibits the same characteristics of the parental cell line.

OVCAR-5/RFP Cell Line

AKR-254 1 vial
EUR 686.4
Description: OVCAR-5/RFP Cell Line stably expresses RFP and otherwise exhibits the same characteristics of the parental cell line.

PKCa Stable Expressing MCF-7 (MCF-7/PKCa20) Cell Line

T6115 1x10^6 cells / 1.0 ml
EUR 3950

Platinum-E Retroviral Packaging Cell Line, Ecotropic

RV-101 1 vial
EUR 770

Platinum-GP Retroviral Packaging Cell Line, Pantropic

RV-103 1 vial
EUR 770

Platinum-A Retroviral Packaging Cell Line, Amphotropic

RV-102 1 vial
EUR 770

Total Protein - Murine Embryonic Stem Cell Line D3

CBA-305 500 ?g
EUR 414
Description:
  • Isolated from mouse ES-D3 cell line
  • Presented as 500 µg at 1 mg/mL in NP-40 Solubilization Buffer

MCF 10A Cell Line

CL-0525 1×10⁶ cells/vial
EUR 500
Description: Homo sapiens, Human

cDNA - Human Tumor Cell Line: MCF 7

C1255830 40 reactions
EUR 424

Flavopiridol-Resistant MCF-7 Cell Line (FLV100)

T8033 1x10^6 cells / 1.0 ml
EUR 2950

Flavopiridol-Resistant MCF-7 Cell Line (FLV1000)

T8035 1x10^6 cells / 1.0 ml Ask for price

Total RNA - Human Tumor Cell Line: MCF 7

R1255830-50 50 ug
EUR 229

Genomic DNA - Human Tumor Cell Line: MCF 7

D1255830 100 ug
EUR 282

Total Protein - Human Tumor Cell Line: MCF 7

P1255830 1 mg
EUR 216

Membrane Protein - Human Tumor Cell Line: MCF 7

P3255830 0.1 mg
EUR 311

Paraffin Tissue Section - Human Tumor Cell Line: MCF-7

T2255830 5 slides
EUR 262

Luc-Akt-PH/YFP Stable MCF-7/B2 Stable Cell Line

T3161 1x10^6 cells / 1.0 ml
EUR 3950

StemTAG Stem Cell Colony Formation Assay (Cell Recovery Compatible)

CBA-325 96 assays
EUR 1027.2
Description: Our StemTAG 96-Well Stem Cell Colony Formation Assay provides a high-throughput method to quantify ES cells in just 7-10 days, and no manual cell counting is required. Once colonies are formed, they may be analyzed in three different ways: 1. Lyse cells, then quantify in a fluorescence plate reader using dye included in the kit; 2. Lyse cells, then quantify alkaline phosphatase activity using reagents provided; or 3. Recover colonies from matrix for further culture or analysis.

StemTAG Stem Cell Colony Formation Assay (Cell Recovery Compatible)

CBA-325-5 5 x 96 assays
EUR 4033.2
Description: Our StemTAG 96-Well Stem Cell Colony Formation Assay provides a high-throughput method to quantify ES cells in just 7-10 days, and no manual cell counting is required. Once colonies are formed, they may be analyzed in three different ways: 1. Lyse cells, then quantify in a fluorescence plate reader using dye included in the kit; 2. Lyse cells, then quantify alkaline phosphatase activity using reagents provided; or 3. Recover colonies from matrix for further culture or analysis.

CytoSelect™ Cell Transformation Assay (Cell Recovery Compatible), Colorimetric

CBA-135 96 assays
EUR 715

CytoSelect™ Cell Transformation Assay (Cell Recovery Compatible), Colorimetric

CBA-135-5 5 x 96 assays
EUR 3095

CytoSelect™ Cell Transformation Assay (Cell Recovery Compatible), Fluorometric

CBA-140 96 assays
EUR 750

CytoSelect™ Cell Transformation Assay (Cell Recovery Compatible), Fluorometric

CBA-140-5 5 x 96 assays
EUR 3215

CytoSelect Cell Transformation Assay (Cell Recovery Compatible), Colorimetric, Trial Size

CBA-135-T 24 assays
EUR 518.4
Description: CytoSelect 96-Well Cell Transformation Assays (Cell Recovery Compatible) provide a robust system for detecting transformed cells, screening cell transformation inhibitors, and determining in vitro drug sensitivity. A proprietary modified soft agar matrix allows you to either quantify cells using the included fluorescent dye, or recover the cells for further analysis.

CytoSelect 96-Well Cell Transformation Assay (Cell Recovery Compatible, Fluorometric), Trial Size

CBA-140-T 24 assays
EUR 547.2
Description: CytoSelect 96-Well Cell Transformation Assays (Cell Recovery Compatible) provide a robust system for detecting transformed cells, screening cell transformation inhibitors, and determining in vitro drug sensitivity. A proprietary modified soft agar matrix allows you to either quantify cells using the included fluorescent dye, or recover the cells for further analysis.

Collagen-based Cell Contraction Assay

CBA-201 24 assays
EUR 420

MCF2L2 (GFP-tagged) - Human MCF.2 cell line derived transforming sequence-like 2 (MCF2L2)

RG212640 10 µg Ask for price

Radius 384-Well Cell Migration Assay

CBA-127 384 assays
EUR 721.2
Description: The Radius Cell Migration Assay provides a unique alternative to conventional cell migration assays using the Boyden chamber. Unlike Boyden chamber assays which may only be analyzed at endpoint, the Radius assay uses a proprietary cell culture plate containing a carefully-defined biocompatible hydrogel (Radius gel) spot centralized at the bottom of each well. When cells are seeded in the well, they will attach everywhere except on the Radius gel, creating a cell-free zone. Following cell seeding the Radius gel is removed, allowing migratory cells to move across the area and close the gap.

Radius 384-Well Cell Migration Assay

CBA-127-5 5 x 384 wells
EUR 2802
Description: The Radius Cell Migration Assay provides a unique alternative to conventional cell migration assays using the Boyden chamber. Unlike Boyden chamber assays which may only be analyzed at endpoint, the Radius assay uses a proprietary cell culture plate containing a carefully-defined biocompatible hydrogel (Radius gel) spot centralized at the bottom of each well. When cells are seeded in the well, they will attach everywhere except on the Radius gel, creating a cell-free zone. Following cell seeding the Radius gel is removed, allowing migratory cells to move across the area and close the gap.

HIF-1 Alpha Cell Based ELISA Kit

CBA-281 96 assays
EUR 734.4
Description: Cell Biolabs? HIF-1 Cell Based ELISA Kit is an immunoassay developed for rapid detection of HIF-1 Alpha in fixed cells. Cells on a microplate are stimulated for HIF-1 Alpha stabilization, fixed, permeabilized, and then neutralized in the well. HIF-1 Alpha is then detected with an anti-HIF-1 alpha antibody followed by an HRP conjugated secondary antibody. Each kit provides sufficient reagents to perform up to a total of 96 assays and can detect HIF-1 Alpha from human, mouse, or rat.

CytoSelect™ MTT Cell Proliferation Assay

CBA-252 960 assays
EUR 320

Radius™ 24-Well Cell Migration Assay

CBA-125 24 assays
EUR 425

Radius™ 24-Well Cell Migration Assay

CBA-125-5 5 x 24 assays
EUR 1820

Radius™ 96-Well Cell Migration Assay

CBA-126 96 assays
EUR 495

Radius™ 96-Well Cell Migration Assay

CBA-126-5 5 x 96 assays
EUR 2095

Radius™ 48-Well Cell Migration Assay

CBA-5037 48 assays
EUR 445

Radius™ 48-Well Cell Migration Assay

CBA-5037-5 5 x 48 assays
EUR 1895

CytoSelect™ BrdU Cell Proliferation ELISA Kit

CBA-251 96 assays
EUR 455

CytoSelect Cell Proliferation Assay Reagent (Fluorometric)

CBA-250 10 mL
EUR 490.8
Description: Cell Biolabs? CytoSelect Cell Proliferation Assay Reagent (Fluorometric) provides a fluorometric format for measuring and monitoring cell proliferation. Cells can be plated and then treated with compounds or agents that affect proliferation.  Cells are then incubated with the proliferation reagent.  Upon entering metabolically active live cells, the non-fluorescent proliferation reagent is converted into a bright red fluorescent form. An increase in cell proliferation is accompanied by increased fluorescent signal, while a decrease in cell proliferation (and signal) can indicate the toxic effects of compounds or suboptimal culture conditions.  The assay principles are basic and can be applied to most eukaryotic cell lines, including adherent and non-adherent cells and certain tissues.  This cell proliferation reagent can be used to detect proliferation in bacteria, yeast, fungi, protozoa as well as cultured mammalian and piscine cells. The kit contains sufficient reagents for the evaluation of 960 assays in ten 96-well plates or 192 assays in eight 24-well plates.

CytoSelect™ 96-well Cell Transformation Assay

CBA-130 96 assays
EUR 640

CytoSelect™ 96-well Cell Transformation Assay

CBA-130-5 5 x 96 assays
EUR 2695

MCF-7 Human breast cancer, noninvasive cell line: >1x10^10 frozen exosomes

EXOP-100A-1 50 ug
EUR 409

CytoSelect™ Cell Viability and Cytotoxicity Assay

CBA-240 96 assays
EUR 305

CytoSelect™ 24-Well Cell Co-Culture System

CBA-160 24 assays
EUR 365

CytoSelect™ 24-Well Cell Co-Culture System

CBA-160-5 5 x 24 assays
EUR 1585

CytoSelect 24-well Laminin Cell Invasion, Colorimetric

CBA-110-LN 12 assays
EUR 714
Description: The ability of malignant tumor cells to invade normal surrounding tissue contributes in large part to the morbidity and mortality of cancers. Cell invasion requires several distinct cellular functions including adhesion, motility, detachment, and extracellular matrix proteolysis. Our CytoSelect Cell Invasion Assays utilize precoated inserts to assay the invasive properties of tumor cells. Invasive cells can be quantified in 24-well plates on either a standard microplate reader or a fluorescence plate reader. Inserts are precoated on the top of the membrane with Laminin.

CytoSelect™ 48-Well Cell Contraction Assay Kit

CBA-5021 48 assays
EUR 575

CytoSelect 384-well Cell Transformation Assay, Fluorometric

CBA-145 384 assays
EUR 1208.4
Description: Our CytoSelect 384-Well Cell Transformation Assay uses a modified soft agar 3D matrix to support the formation of colonies by neoplastic cells. Quantitation of cell transformation is performed on a fluorescence plate reader.

CytoSelect 384-well Cell Transformation Assay, Fluorometric

CBA-145-5 5 x 384 assays
EUR 4681.2
Description: Our CytoSelect 384-Well Cell Transformation Assay uses a modified soft agar 3D matrix to support the formation of colonies by neoplastic cells. Quantitation of cell transformation is performed on a fluorescence plate reader.

CytoSelect™ Cell Proliferation Assay Reagent (Colorimetric)

CBA-253 10 mL
EUR 320

Radius 24-Well Cell Migration Assay, (Laminin Coated)

CBA-125-LN 24 assays
EUR 714
Description: The Radius Cell Migration Assay provides a unique alternative to conventional cell migration assays using the Boyden chamber. Unlike Boyden chamber assays which may only be analyzed at endpoint, the Radius assay uses a proprietary cell culture plate containing a carefully-defined biocompatible hydrogel (Radius gel) spot centralized at the bottom of each well. When cells are seeded in the well, they will attach everywhere except on the Radius gel, creating a cell-free zone. Following cell seeding the Radius gel is removed, allowing migratory cells to move across the area and close the gap.

MCF2 (GFP-tagged) - Human MCF.2 cell line derived transforming sequence (MCF2), transcript variant 4

RG230516 10 µg Ask for price

MCF2 (GFP-tagged) - Human MCF.2 cell line derived transforming sequence (MCF2), transcript variant 5

RG230549 10 µg Ask for price

MCF2 (GFP-tagged) - Human MCF.2 cell line derived transforming sequence (MCF2), transcript variant 6

RG230587 10 µg Ask for price

MCF2 (GFP-tagged) - Human MCF.2 cell line derived transforming sequence (MCF2), transcript variant 3

RG230610 10 µg Ask for price

MCF2 (GFP-tagged) - Human MCF.2 cell line derived transforming sequence (MCF2), transcript variant 1

RG212933 10 µg Ask for price

MCF2 (GFP-tagged) - Human MCF.2 cell line derived transforming sequence (MCF2), transcript variant 2

RG218217 10 µg Ask for price

StemTAG PCR Primer Set for Stem Cell Characterization

CBA-303 1 kit
EUR 470.4
Description: StemTAG PCR Primer Set for Stem Cell Characterization includes 7 primer pairs: Oct-4, NANOG, AFP, Flk-1, and NCAM, plus GAPDH and beta-actin as controls.

CytoSelect Clonogenic Tumor Cell Isolation Kit (5 preps)

CBA-155 5 preps
EUR 957.6
Description: Many solid tumors contain heterogeneous populations of normal and cancerous cells. Separation of these cell populations is key to an accurate assessment of the true genotypic and phenotypic differences between normal and tumor cells. Our CytoSelect Clonogenic Tumor Cell Isolation Kit uses a proprietary semisolid agar medium to facilitate formation of colonies by cells from solid tumors. Colonies are grown in either a 6-well plate or a 35mm culture dish. These colonies are isolated away from single (i.e. normal) cells by size filtration. The viable cells from these colonies can be easily recovered for further analysis.

Radius 24-Well Cell Migration Assay, (Fibronectin Coated)

CBA-125-FN 24 assays
EUR 714
Description: The Radius Cell Migration Assay provides a unique alternative to conventional cell migration assays using the Boyden chamber. Unlike Boyden chamber assays which may only be analyzed at endpoint, the Radius assay uses a proprietary cell culture plate containing a carefully-defined biocompatible hydrogel (Radius gel) spot centralized at the bottom of each well. When cells are seeded in the well, they will attach everywhere except on the Radius gel, creating a cell-free zone. Following cell seeding the Radius gel is removed, allowing migratory cells to move across the area and close the gap.

CytoSelect™ 24-well Collagen Cell Invasion, Colorimetric

CBA-110-COL 12 assays
EUR 535

Radius 24-Well Cell Migration Assay, (ECM Array Coated)

CBA-125-ECM 24 wells
EUR 838.8
Description: The Radius Cell Migration Assay provides a unique alternative to conventional cell migration assays using the Boyden chamber. Unlike Boyden chamber assays which may only be analyzed at endpoint, the Radius assay uses a proprietary cell culture plate containing a carefully-defined biocompatible hydrogel (Radius gel) spot centralized at the bottom of each well. When cells are seeded in the well, they will attach everywhere except on the Radius gel, creating a cell-free zone. Following cell seeding the Radius gel is removed, allowing migratory cells to move across the area and close the gap.

CytoSelect Proliferating Cell Nuclear Antigen (PCNA) ELISA Kit

CBA-254 96 assays
EUR 672
Description: Cell Biolabs? CytoSelect Proliferating Cell Nuclear Antigen (PCNA) ELISA Kit is an enzyme immunoassay developed for the detection and quantitation of PCNA from nuclear and whole cell extracts.  The kit detects PCNA from mouse, rat and human, and has a detection sensitivity limit of 12.5 ng/mLPCNA.  Each kit provides sufficient reagents to perform up to 96 assays including standard curve and unknown samples. 

CytoSelect 48-well Cell Adhesion Assay (Laminin, Colorimetric)

CBA-056 48 assays
EUR 512.4
Description: Cell adhesion is a complex process involved in migration/invasion, embryogenesis, wound healing and tissue remodeling. Cells adhere to the extracellular matrix, forming complexes with cytoskeleton components that can affect cell motility, differentiation, proliferation, and survival. Our CytoSelect 48-Well Cell Adhesion Assays provide a fully quantitative method for the evaluation of cell adhesion. The 48-well plate is precoated with Laminin.

CytoSelect 48-well Cell Adhesion Assay (Laminin, Fluorometric)

CBA-057 48 assays
EUR 553.2
Description: Cell adhesion is a complex process involved in migration/invasion, embryogenesis, wound healing and tissue remodeling. Cells adhere to the extracellular matrix, forming complexes with cytoskeleton components that can affect cell motility, differentiation, proliferation, and survival. Our CytoSelect 48-Well Cell Adhesion Assays provide a fully quantitative method for the evaluation of cell adhesion. The 48-well plate is precoated with Laminin.

CytoSelect 24-well Laminin Cell Invasion Assay, Fluorometric

CBA-111-LN 12 assays
EUR 714
Description: The ability of malignant tumor cells to invade normal surrounding tissue contributes in large part to the morbidity and mortality of cancers. Cell invasion requires several distinct cellular functions including adhesion, motility, detachment, and extracellular matrix proteolysis. Our CytoSelect Cell Invasion Assays utilize precoated inserts to assay the invasive properties of tumor cells. Invasive cells can be quantified in 24-well plates on either a standard microplate reader or a fluorescence plate reader. Inserts are precoated on the top of the membrane with Laminin.

CytoSelect 96-well Laminin Cell Invasion Assay, Fluorometric

CBA-112-LN 96 assays
EUR 908.4
Description: The ability of malignant tumor cells to invade normal surrounding tissue contributes in large part to the morbidity and mortality of cancers. Cell invasion requires several distinct cellular functions including adhesion, motility, detachment, and extracellular matrix proteolysis. Our CytoSelect 96-Well Cell Invasion Assays utilize precoated inserts to assay the invasive properties of tumor cells. Invasive cells can be quantified in 96-well plates on a fluorescence plate reader. Inserts are precoated on the top of the membrane with Laminin.

CytoSelect 48-well Cell Adhesion Assay (Fibrinogen, Colorimetric)

CBA-058 48 assays
EUR 512.4
Description: Cell adhesion is a complex process involved in migration/invasion, embryogenesis, wound healing and tissue remodeling. Cells adhere to the extracellular matrix, forming complexes with cytoskeleton components that can affect cell motility, differentiation, proliferation, and survival. Our CytoSelect 48-Well Cell Adhesion Assays provide a fully quantitative method for the evaluation of cell adhesion. The 48-well plate is precoated with Fibrinogen.

CytoSelect 48-well Cell Adhesion Assay (Fibrinogen, Fluorometric)

CBA-059 48 assays
EUR 553.2
Description: Cell adhesion is a complex process involved in migration/invasion, embryogenesis, wound healing and tissue remodeling. Cells adhere to the extracellular matrix, forming complexes with cytoskeleton components that can affect cell motility, differentiation, proliferation, and survival. Our CytoSelect 48-Well Cell Adhesion Assays provide a fully quantitative method for the evaluation of cell adhesion. The 48-well plate is precoated with Fibrinogen.

CytoSelect™ 96-well Phagocytosis Assay (Red Blood Cell)

CBA-220 96 assays
EUR 540

GFP ELISA Kit

AKR-121 96 assays
EUR 665

GFP ELISA Kit

AKR-121-5 5 x 96 assays
EUR 2930

Radius™ 24-Well Cell Migration Assay, (Collagen I Coated)

CBA-125-COL 24 assays
EUR 520

CytoSelect™ 24-well Collagen Cell Invasion Assay, Fluorometric

CBA-111-COL 12 assays
EUR 535

CytoSelect™ 96-well Collagen Cell Invasion Assay, Fluorometric

CBA-112-COL 96 assays
EUR 705

CytoSelect Clonogenic Tumor Cell Isolation Kit (5 x 5 preps)

CBA-155-5 25 preps
EUR 3894
Description: Many solid tumors contain heterogeneous populations of normal and cancerous cells. Separation of these cell populations is key to an accurate assessment of the true genotypic and phenotypic differences between normal and tumor cells. Our CytoSelect Clonogenic Tumor Cell Isolation Kit uses a proprietary semisolid agar medium to facilitate formation of colonies by cells from solid tumors. Colonies are grown in either a 6-well plate or a 35mm culture dish. These colonies are isolated away from single (i.e. normal) cells by size filtration. The viable cells from these colonies can be easily recovered for further analysis.

CytoSelect™ 48-well Cell Adhesion Assay (Fibronectin, Colorimetric)

CBA-050 48 assays
EUR 370

CytoSelect™ 48-well Cell Adhesion Assay (Fibronectin, Fluorometric)

CBA-051 48 assays
EUR 400

CytoSelect™ 24-well Cell Invasion (Basement Membrane), Fluorometric

CBA-111 12 assays
EUR 535

GFP Quantitation Kit

AKR-120 100 assays
EUR 495

CytoSelect™ 24-well Cell Migration Assay (8 μm), Colorimetric

CBA-100 12 assays
EUR 480

CytoSelect™ 24-well Cell Migration Assay (8 μm), Colorimetric

CBA-100-5 5 x 12 assays
EUR 2180

CytoSelect™ 24-well Cell Migration Assay (8 μm), Fluorometric

CBA-101 12 assays
EUR 480

CytoSelect™ 24-well Cell Migration Assay (8 μm), Fluorometric

CBA-101-5 5 x 12 assays
EUR 2180

CytoSelect™ 24-well Cell Migration Assay (5 μm), Fluorometric

CBA-102 12 assays
EUR 480

CytoSelect™ 24-well Cell Migration Assay (5 μm), Fluorometric

CBA-102-5 5 x 12 assays
EUR 2180

CytoSelect™ 24-well Cell Migration Assay (3 μm), Fluorometric

CBA-103 12 assays
EUR 480

CytoSelect™ 24-well Cell Migration Assay (3 μm), Fluorometric

CBA-103-5 5 x 12 assays
EUR 2180

CytoSelect™ 96-well Cell Migration Assay (3 μm), Fluorometric

CBA-104 96 assays
EUR 580

CytoSelect™ 96-well Cell Migration Assay (3 μm), Fluorometric

CBA-104-5 5 x 96 assays
EUR 2535

CytoSelect™ 96-well Cell Migration Assay (5 μm), Fluorometric

CBA-105 96 assays
EUR 580

CytoSelect™ 96-well Cell Migration Assay (5 μm), Fluorometric

CBA-105-5 5 x 96 assays
EUR 2535

CytoSelect™ 96-well Cell Migration Assay (8 μm), Fluorometric

CBA-106 96 assays
EUR 580

CytoSelect™ 96-well Cell Migration Assay (8 μm), Fluorometric

CBA-106-5 5 x 96 assays
EUR 2535

CytoSelect™ 24-well Cell Migration Assay (12 μm), Colorimetric

CBA-107 12 assays
EUR 480

CytoSelect™ 24-well Cell Migration Assay (12 μm), Fluorometric

CBA-108 12 assays
EUR 480

GFP Lentivirus Control

LTV-300 1 vial
EUR 455

CytoSelect™ 48-well Cell Adhesion Assay (ECM Array, Colorimetric)

CBA-070 48 assays
EUR 515

CytoSelect™ 48-well Cell Adhesion Assay (ECM Array, Colorimetric)

CBA-070-5 5 x 48 assays
EUR 2275

CytoSelect™ 48-well Cell Adhesion Assay (ECM Array, Fluorometric)

CBA-071 48 assays
EUR 555

CytoSelect™ 48-well Cell Adhesion Assay (ECM Array, Fluorometric)

CBA-071-5 5 x 48 assays
EUR 2430

GFP Recombinant Adenovirus

ADV-004 50 ?L
EUR 745

CytoSelect™ 48-well Cell Adhesion Assay (Collagen I, Colorimetric)

CBA-052 48 assays
EUR 370

CytoSelect™ 48-well Cell Adhesion Assay (Collagen I, Fluorometric)

CBA-053 48 assays
EUR 400

CytoSelect™ 48-well Cell Adhesion Assay (Collagen IV, Colorimetric)

CBA-060 48 assays
EUR 370

CytoSelect™ 48-well Cell Adhesion Assay (Collagen IV, Fluorometric)

CBA-061 48 assays
EUR 400

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